Effects of New Mutations in BMPRIB, GDF9, BMP15, LEPR, and B4GALNT2 Genes on Litter Size in Sheep
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. SNP Genotyping by iPLEX MassARRAY
2.3. Statistical Analyses
3. Results
3.1. Genetic Diversity Analysis
3.2. Associations between Mutations in Candidate Genes and Litter Size in Sheep
3.2.1. Associations between Mutations in BMPR1B and Litter Size in Sheep
3.2.2. Associations between Mutations in GDF9 and Litter Size in Sheep
3.2.3. Associations between Mutations in BMP15 and Litter Size in Sheep
3.2.4. Associations between Mutations in LEPR and Litter Size in Sheep
3.2.5. Associations between Mutations in B4GALNT2 and Litter Size in Sheep
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Breed | Abbreviation | Number of Ewes | Type |
---|---|---|---|
Ujimqin | UM | 325 (single lamb 264 + twin lambs 61) | Single birth |
Dorper × Ujimqin F1 | DPU | 304 (single lamb 180 + twin lambs 124) | Single birth |
Suffolk × Ujimqin F1 | SFKU | 66 (single lamb 28 + twin lambs 38) | Single birth |
Mongolia 1 | MG | 250 | Single birth |
Sonid | SN | 184 | Single birth |
Tan | Tan | 30 | Single birth |
Hu | Hu | 30 | Multiple births |
Small-tailed Han | STH | 30 | Multiple births |
Sheep Population | Mutation | Genotype | Number | Litter Size |
---|---|---|---|---|
Ujimqin | c.746A>G (Gln249Arg) | AA | 320 | 1.18 ± 0.02 a |
AG | 5 | 1.60 ± 0.24 b | ||
c.1470G>T (490Thr) | AA | 45 | 1.20 ± 0.05 | |
AC | 155 | 1.17 ± 0.03 | ||
CC | 116 | 1.21 ± 0.04 | ||
Dorper × Ujimqin F1 | c.746A>G (Gln249Arg) | AA | 300 | 1.40 ± 0.03 a |
AG | 4 | 2.00 ± 0.00 b | ||
c.1470G>T (490Thr) | AA | 12 | 1.25 ± 0.13 | |
AC | 111 | 1.41± 0.05 | ||
CC | 181 | 1.42 ± 0.04 | ||
Suffolk × Ujimqin F1 | c.1470G>T (490Thr) | AA | 14 | 1.50 ± 0.14 |
AC | 40 | 1.63 ± 0.08 | ||
CC | 12 | 1.28 ± 0.06 |
Breed | Mutation | Genotype | Number | Litter Size |
---|---|---|---|---|
Ujimqin | g.46547859C>T | CC | 244 | 1.19 ± 0.03 |
CT | 79 | 1.18 ± 0.04 | ||
g.46547645T>G | TT | 198 | 1.20 ± 0.03 | |
TG | 118 | 1.16 ± 0.03 | ||
c.260G>A (Arg87His) | GG | 292 | 1.19 ± 0.02 | |
GA | 32 | 1.16 ± 0.07 | ||
c.994A>G (Val322Ile) | AA | 299 | 1.19 ± 0.02 | |
AG | 24 | 1.21 ± 0.08 | ||
g.46544883A>G | AA | 244 | 1.19 ± 0.03 | |
AG | 79 | 1.18 ± 0.04 | ||
Dorper × Ujimqin F1 | g.46547859C>T | CC | 189 | 1.40 ± 0.04 |
CT | 107 | 1.43 ± 0.05 | ||
g.46547645T>G | TT | 178 | 1.40 ± 0.04 | |
GT | 113 | 1.42 ± 0.05 | ||
c.994A>G (Val322Ile) | AA | 279 | 1.41 ± 0.03 | |
AG | 28 | 1.43 ± 0.09 | ||
g.46544883A>G | AA | 189 | 1.40 ± 0.04 | |
AG | 106 | 1.43 ± 0.05 | ||
Suffolk × Ujimqin F1 | g.46547859C>T | CC | 51 | 1.59 ± 0.07 |
CT | 13 | 1.61 ± 0.14 | ||
g.46547645T>G | TT | 48 | 1.60 ± 0.07 | |
TG | 16 | 1.56 ± 0.13 | ||
c.994A>G (Val322Ile) | AA | 46 | 1.48 ± 0.07 a | |
AG | 20 | 1.80 ± 0.09 b | ||
g.46544883A>G | AA | 51 | 1.59 ± 0.07 | |
AG | 13 | 1.62 ± 0.14 |
Breed | Mutation | Genotype | Number | Litter Size |
---|---|---|---|---|
Ujimqin | g.509807863G>A | GG | 287 | 1.19 ± 0.02 |
GA | 38 | 1.18 ± 0.06 | ||
c.31_33CTTinsdel (11Leu deletion) | CCT.CCT | 191 | 1.13 ± 0.02 A | |
CCT.DEL | 112 | 1.27 ± 0.04 B | ||
DEL.DEL | 22 | 1.27 ± 0.10 | ||
c.755T>C (Lue252Pro) | TT | 259 | 1.19 ± 0.02 | |
TC | 64 | 1.20 ± 0.05 | ||
Dorper × Ujimqin F1 | c.31_33CTTinsdel (11Leu deletion) | CCT.CCT | 84 | 1.38 ± 0.05 |
CCT.DEL | 167 | 1.41 ± 0.04 | ||
DEL.DEL | 53 | 1.43 ± 0.07 | ||
c.755T>C (Lue252Pro) | TT | 235 | 1.41 ± 0.03 | |
TC | 66 | 1.41 ± 0.06 | ||
Suffolk × Ujimqin F1 | c.31_33CTTinsdel (11Leu_deletion) | CCT.CCT | 44 | 1.59 ± 0.07 |
CCT.DEL | 20 | 1.60 ± 0.11 |
Breed | Mutation | Genotype | Number | Litter Size |
---|---|---|---|---|
Ujimqin | c.185G>A (Arg62His) | GG | 249 | 1.21 ± 0.03 |
GA | 71 | 1.11 ± 0.04 | ||
c.240C>T (80Asn) | CC | 60 | 1.10 ± 0.04 | |
CT | 147 | 1.18 ± 0.03 | ||
TT | 118 | 1.24 ± 0.04 | ||
c.279C>T (93Ser) | CC | 60 | 1.10 ± 0.04 | |
CT | 147 | 1.18 ± 0.03 | ||
TT | 118 | 1.24 ± 0.04 | ||
Dorper × Ujimqin F1 | c.185G>A (Arg62His) | GG | 249 | 1.41 ± 0.03 |
GA | 55 | 1.40 ± 0.07 | ||
c.240C>T (80Asn) | CC | 16 | 1.38 ± 0.13 | |
CT | 121 | 1.43 ± 0.06 | ||
TT | 167 | 1.40 ± 0.04 | ||
c.279C>T (93Ser) | CC | 16 | 1.38 ± 0.13 | |
CT | 121 | 1.43 ± 0.06 | ||
TT | 167 | 1.40 ± 0.04 | ||
Suffolk × Ujimqin F1 | c.185G>A (Arg62His) | GG | 35 | 1.51 ± 0.09 |
GA | 25 | 1.56 ± 0.10 | ||
c.240C>T (80Asn) | CT | 35 | 1.66 ± 0.08 | |
TT | 26 | 1.46 ± 0.10 | ||
c.279C>T (93Ser) | CT | 35 | 1.66 ± 0.08 | |
TT | 26 | 1.46 ± 0.10 |
Breed | Mutation | Genotype | Number | Litter Size |
---|---|---|---|---|
Ujimqin | c.440C>T (Pro160Leu) | CC | 255 | 1.18 ± 0.02 |
CT | 66 | 1.20 ± 0.05 | ||
g.25929637G>A | GG | 289 | 1.19 ± 0.02 | |
GA | 35 | 1.17 ± 0.06 | ||
g.25929679T>C | TT | 229 | 1.18 ± 0.03 | |
TC | 93 | 1.18 ± 0.04 | ||
g.25929819A>G | AA | 229 | 1.18 ± 0.03 | |
AG | 93 | 1.18 ± 0.04 | ||
g.25929965A>T | AA | 289 | 1.19 ± 0.02 | |
AT | 33 | 1.12 ± 0.06 | ||
c.823C>T (Pro288Ser) | CC | 218 | 1.22 ± 0.03 | |
CT | 93 | 1.14 ± 0.04 | ||
TT | 14 | 1.07 ± 0.07 | ||
Dorper × Ujimqin F1 | c.440C>T (Pro160Leu) | CC | 251 | 1.43 ± 0.03 |
CT | 52 | 1.33 ± 0.07 | ||
g.25929679T>C | TT | 243 | 1.39 ± 0.03 | |
TC | 59 | 1.44 ± 0.07 | ||
g.25929819A>G | AA | 243 | 1.40 ± 0.03 | |
AG | 59 | 1.44 ± 0.07 | ||
c.823C>T (Pro288Ser) | CC | 244 | 1.39 ± 0.03 | |
CT | 57 | 1.49 ± 0.07 | ||
Suffolk × Ujimqin F1 | c.440C>T (Pro160Leu) | CC | 51 | 1.59 ± 0.07 |
CT | 14 | 1.57 ± 0.14 | ||
g.25929679T>C | TT | 20 | 1.40 ± 0.11 | |
TC | 39 | 1.64 ± 0.08 | ||
g.25929819A>G | AA | 20 | 1.40 ± 0.11 | |
AG | 39 | 1.64 ± 0.08 |
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Ji, X.; Cao, Z.; Hao, Q.; He, M.; Cang, M.; Yu, H.; Ma, Q.; Li, X.; Bao, S.; Wang, J.; et al. Effects of New Mutations in BMPRIB, GDF9, BMP15, LEPR, and B4GALNT2 Genes on Litter Size in Sheep. Vet. Sci. 2023, 10, 258. https://doi.org/10.3390/vetsci10040258
Ji X, Cao Z, Hao Q, He M, Cang M, Yu H, Ma Q, Li X, Bao S, Wang J, et al. Effects of New Mutations in BMPRIB, GDF9, BMP15, LEPR, and B4GALNT2 Genes on Litter Size in Sheep. Veterinary Sciences. 2023; 10(4):258. https://doi.org/10.3390/vetsci10040258
Chicago/Turabian StyleJi, Xuewen, Ziwei Cao, Qi Hao, Mei He, Ming Cang, Haiquan Yu, Qing Ma, Xihe Li, Siqin Bao, Jianguo Wang, and et al. 2023. "Effects of New Mutations in BMPRIB, GDF9, BMP15, LEPR, and B4GALNT2 Genes on Litter Size in Sheep" Veterinary Sciences 10, no. 4: 258. https://doi.org/10.3390/vetsci10040258