Next Article in Journal
The Impact of a New “Inverted Arch” Prosthetic Annuloplasty Ring on the Mitral Valve’s 3-D Motion: An Experimental Ex-Vivo Study
Previous Article in Journal
QbD Based Media Development for the Production of Fab Fragments in E. coli
Previous Article in Special Issue
Thermoplastic PCL-b-PEG-b-PCL and HDI Polyurethanes for Extrusion-Based 3D-Printing of Tough Hydrogels
Article Menu

Export Article

Open AccessArticle

3D Encapsulation Made Easy: A Coaxial-Flow Circuit for the Fabrication of Hydrogel Microfibers Patches

1
Politecnico di Milano, Department of Chemistry, Materials and Chemical Engineering “G. Natta”, Via Mancinelli 7, 20131 Milano, Italy
2
INSTM—National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti, 9-50121 Firenze, Italy
3
Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy
*
Author to whom correspondence should be addressed.
Bioengineering 2019, 6(2), 30; https://doi.org/10.3390/bioengineering6020030
Received: 26 February 2019 / Revised: 23 March 2019 / Accepted: 3 April 2019 / Published: 6 April 2019
(This article belongs to the Special Issue Applying Polymeric Biomaterials in 3D Tissue Constructs)
  |  
PDF [2326 KB, uploaded 6 April 2019]
  |  

Abstract

To fully exploit the potential of hydrogel micro-fibers in the design of regenerative medicinal materials, we designed a simple, easy to replicate system for cell embedding in degradable fibrous scaffolds, and validated its effectiveness using alginate-based materials. For scaffold fabrication, cells are suspended in a hydrogel-precursor and injected in a closed-loop circuit, where a pump circulates the ionic cross-linking solution. The flow of the cross-linking solution stretches and solidifies a continuous micro-scaled, cell-loaded hydrogel fiber that whips, bends, and spontaneously assembles in a self-standing, spaghetti-like patch. After investigation and tuning of process- and solution-related parameters, homogeneous microfibers with controlled diameters and consistent scaffolds were obtained from different alginate concentrations and blends with biologically favorable macromolecules (i.e., gelatin or hyaluronic acid). Despite its simplicity, this coaxial-flow encapsulation system allows for the rapid and effortless fabrication of thick, well-defined scaffolds, with viable cells being homogeneously distributed within the fibers. The reduced fiber diameter and the inherent macro-porous structure that is created from the random winding of fibers can sustain mass transport, and support encapsulated cell survival. As different materials and formulations can be processed to easily create homogeneously cell-populated structures, this system appears as a valuable platform, not only for regenerative medicine, but also, more in general, for 3D cell culturing in vitro.
View Full-Text
Keywords: degradable hydrogels; cell delivery; cell encapsulation; microfibers scaffolds; calcium alginate; gelatin; hyaluronic acid degradable hydrogels; cell delivery; cell encapsulation; microfibers scaffolds; calcium alginate; gelatin; hyaluronic acid
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Campiglio, C.E.; Ceriani, F.; Draghi, L. 3D Encapsulation Made Easy: A Coaxial-Flow Circuit for the Fabrication of Hydrogel Microfibers Patches. Bioengineering 2019, 6, 30.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Bioengineering EISSN 2306-5354 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top