Next Article in Journal
Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion
Next Article in Special Issue
Comparison of the Zebrafish Embryo Toxicity Assay and the General and Behavioral Embryo Toxicity Assay as New Approach Methods for Chemical Screening
Previous Article in Journal / Special Issue
Development of a Larval Zebrafish Model for Acute Organophosphorus Nerve Agent and Pesticide Exposure and Therapeutic Evaluation
Article

An Embryonic Zebrafish Model to Screen Disruption of Gut-Vascular Barrier upon Exposure to Ambient Ultrafine Particles

1
Department of Bioengineering and Medicine, University of California, Los Angeles, CA 90095, USA
2
Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310027, China
3
Department of Civil and Environmental Engineering, University of Southern California, Los Angeles, CA 90089, USA
4
College of Health Sciences and Environmental Engineering, Shenzhen Technology University, Shenzhen 518118, China
5
Division of Cardiology, Department of Medicine, School of Medicine, University of California, Los Angeles, CA 90095, USA
6
Veterans Affairs Greater Los Angeles Healthcare System, Department of Medicine, Los Angeles, CA 90073, USA
*
Author to whom correspondence should be addressed.
Equally contributed.
Toxics 2020, 8(4), 107; https://doi.org/10.3390/toxics8040107
Received: 29 October 2020 / Revised: 16 November 2020 / Accepted: 17 November 2020 / Published: 19 November 2020
(This article belongs to the Special Issue Advanced Zebrafish Model for Environmental Health Sciences Research)
Epidemiological studies have linked exposure to ambient particulate matter (PM) with gastrointestinal (GI) diseases. Ambient ultrafine particles (UFP) are the redox-active sub-fraction of PM2.5, harboring elemental and polycyclic aromatic hydrocarbons from urban environmental sources including diesel and gasoline exhausts. The gut-vascular barrier (GVB) regulates paracellular trafficking and systemic dissemination of ingested microbes and toxins. Here, we posit that acute UFP ingestion disrupts the integrity of the intestinal barrier by modulating intestinal Notch activation. Using zebrafish embryos, we performed micro-gavage with the fluorescein isothiocynate (FITC)-conjugated dextran (FD10, 10 kDa) to assess the disruption of GVB integrity upon UFP exposure. Following micro-gavage, FD10 retained in the embryonic GI system, migrated through the cloaca. Conversely, co-gavaging UFP increased transmigration of FD10 across the intestinal barrier, and FD10 fluorescence occurred in the venous capillary plexus. Ingestion of UFP further impaired the mid-intestine morphology. We performed micro-angiogram of FD10 to corroborate acute UFP-mediated disruption of GVB. Transient genetic and pharmacologic manipulations of global Notch activity suggested Notch regulation of the GVB. Overall, our integration of a genetically tractable embryonic zebrafish and micro-gavage technique provided epigenetic insights underlying ambient UFP ingestion disrupts the GVB. View Full-Text
Keywords: ultrafine particles; zebrafish; micro-gavage; Notch signaling; gut-vascular barrier ultrafine particles; zebrafish; micro-gavage; Notch signaling; gut-vascular barrier
Show Figures

Graphical abstract

MDPI and ACS Style

Baek, K.I.; Qian, Y.; Chang, C.-C.; O’Donnell, R.; Soleimanian, E.; Sioutas, C.; Li, R.; Hsiai, T.K. An Embryonic Zebrafish Model to Screen Disruption of Gut-Vascular Barrier upon Exposure to Ambient Ultrafine Particles. Toxics 2020, 8, 107. https://doi.org/10.3390/toxics8040107

AMA Style

Baek KI, Qian Y, Chang C-C, O’Donnell R, Soleimanian E, Sioutas C, Li R, Hsiai TK. An Embryonic Zebrafish Model to Screen Disruption of Gut-Vascular Barrier upon Exposure to Ambient Ultrafine Particles. Toxics. 2020; 8(4):107. https://doi.org/10.3390/toxics8040107

Chicago/Turabian Style

Baek, Kyung I., Yi Qian, Chih-Chiang Chang, Ryan O’Donnell, Ehsan Soleimanian, Constantinos Sioutas, Rongsong Li, and Tzung K. Hsiai 2020. "An Embryonic Zebrafish Model to Screen Disruption of Gut-Vascular Barrier upon Exposure to Ambient Ultrafine Particles" Toxics 8, no. 4: 107. https://doi.org/10.3390/toxics8040107

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop