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Article

Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion

1
Division of Basic Research, Koo Foundation Sun Yat-Sen Cancer Center, Taipei 112, Taiwan
2
Department of Chemistry, Tamkang University, Tamsui, New Taipei City 251, Taiwan
*
Author to whom correspondence should be addressed.
Toxics 2020, 8(4), 108; https://doi.org/10.3390/toxics8040108
Received: 6 October 2020 / Revised: 13 November 2020 / Accepted: 18 November 2020 / Published: 20 November 2020
(This article belongs to the Section Toxicology)
(1) Background: Amikacin is an aminoglycoside antibiotic used for treating gram-negative bacterial infections in cancer patients. In this study, our aims are to investigate the migratory inhibition effects of amikacin in human MDA-MB-231 cells. (2) Methods: We used a wound-healing assay, trans-well analysis, Western blotting, immunostaining and siRNA knockdown approaches to investigate how amikacin influenced MDA-MB-231 cell migration and invasion. (3) Results: Wound healing showed that the MDA-MB-231 cell migration rates decreased to 44.4% in the presence of amikacin. Trans-well analysis showed that amikacin treatment led to invasion inhibition. Western blotting demonstrated that amikacin induced thioredoxin-interacting protein (TXNIP) up-regulation. TXNIP was knocked down using siRNA in MDA-MB-231 cell. Using immunostaining analysis, we found that inhibition of TXNIP expression led to MDA-MB-231 pseudopodia extension; however, amikacin treatment attenuated the cell extension formation. (4) Conclusions: We observed inhibition of migration and invasion in MDA-MB-231 cells treated with amikacin. This suggests inhibition might be mediated by up-regulation of TXNIP. View Full-Text
Keywords: amikacin; TXNIP; siRNA; breast cancer cells amikacin; TXNIP; siRNA; breast cancer cells
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MDPI and ACS Style

Wang, Y.-H.; Chen, Y.-H.; Shen, W.-H. Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion. Toxics 2020, 8, 108. https://doi.org/10.3390/toxics8040108

AMA Style

Wang Y-H, Chen Y-H, Shen W-H. Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion. Toxics. 2020; 8(4):108. https://doi.org/10.3390/toxics8040108

Chicago/Turabian Style

Wang, Yun-Hsin, Yau-Hung Chen, and Wen-Hao Shen. 2020. "Amikacin Suppresses Human Breast Cancer Cell MDA-MB-231 Migration and Invasion" Toxics 8, no. 4: 108. https://doi.org/10.3390/toxics8040108

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