Metabolomic Screening of Anti-Inflammatory Compounds from the Leaves of Actinidia arguta (Hardy Kiwi)
Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea
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Author to whom correspondence should be addressed.
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These authors contribute to this work equally.
Foods 2019, 8(2), 47; https://doi.org/10.3390/foods8020047
Received: 26 December 2018
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Revised: 23 January 2019
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Accepted: 24 January 2019
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Published: 1 February 2019
(This article belongs to the Special Issue Phytochemicals in Food and Health)
The metabolomic screening of potential anti-inflammatory compounds in the leaves of Actinidia arguta was performed by using LC-MS/MS. Ethanol extracts were prepared, and the anti-inflammatory effects were investigated based on nitric oxide (NO) synthesis and inducible nitric oxide synthase expression in lipopolysaccharide-induced RAW 264.7 macrophages. The 75% ethanol extract showed the highest inhibitory effect on nitric oxide (NO) production, and it was further separated by in vitro bioassay-guided fractionation using preparative LC with reversed-phase column separation. Through multiple steps of fractionation, sub-fraction 1-3 was finally purified, and caffeic acid derivatives, such as caffeoylthreonic acid and danshensu (salvianic acid A), were successfully identified as key anti-inflammatory compounds by LC-MS/MS and metabolomics analyses. This is the first study identifying anti-inflammatory compounds in A. arguta (Actinidia arguta) leaves through bioassay-guided fractionation and metabolomics screening. Results of this study would be useful for the application of A. arguta leaves as a nutraceutical.
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MDPI and ACS Style
Kim, G.-D.; Lee, J.Y.; Auh, J.-H. Metabolomic Screening of Anti-Inflammatory Compounds from the Leaves of Actinidia arguta (Hardy Kiwi). Foods 2019, 8, 47. https://doi.org/10.3390/foods8020047
AMA Style
Kim G-D, Lee JY, Auh J-H. Metabolomic Screening of Anti-Inflammatory Compounds from the Leaves of Actinidia arguta (Hardy Kiwi). Foods. 2019; 8(2):47. https://doi.org/10.3390/foods8020047
Chicago/Turabian StyleKim, Gyoung-Deuck, Jin Y. Lee, and Joong-Hyuck Auh. 2019. "Metabolomic Screening of Anti-Inflammatory Compounds from the Leaves of Actinidia arguta (Hardy Kiwi)" Foods 8, no. 2: 47. https://doi.org/10.3390/foods8020047
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