Next Article in Journal / Special Issue
Optoacoustic Calcium Imaging of Deep Brain Activity in an Intracardially Perfused Mouse Brain Model
Previous Article in Journal
Photonic and Iontronic Sensing in GaInAsP Semiconductor Photonic Crystal Nanolasers
Open AccessReview

A Review of Intrinsic Optical Imaging Serial Blockface Histology (ICI-SBH) for Whole Rodent Brain Imaging

1
Big Data Institute, Institute of Biomedical Engineering, University of Oxford, Oxford OX3 7LF, UK
2
Institute of Biomedical Engineering, École Polytechnique de Montréal, Montréal, QC H3T 1J4, Canada
3
Research Center, Montreal Heart Institute, Montréal, QC H1T 1C8, Canada
*
Authors to whom correspondence should be addressed.
Photonics 2019, 6(2), 66; https://doi.org/10.3390/photonics6020066
Received: 30 April 2019 / Revised: 6 June 2019 / Accepted: 7 June 2019 / Published: 11 June 2019
(This article belongs to the Special Issue Neurophotonics – Optics for the Brain)
In recent years, multiple serial histology techniques were developed to enable whole rodent brain imaging in 3-D. The main driving forces behind the emergence of these imaging techniques were the genome-wide atlas of gene expression in the mouse brain, the pursuit of the mouse brain connectome, and the BigBrain project. These projects rely on the use of optical imaging to target neuronal structures with histological stains or fluorescent dyes that are either expressed by transgenic mice or injected at specific locations in the brain. Efforts to adapt the serial histology acquisition scheme to use intrinsic contrast imaging (ICI) were also put forward, thus leveraging the natural contrast of neuronal tissue. This review focuses on these efforts. First, the origin of optical contrast in brain tissue is discussed with emphasis on the various imaging modalities exploiting these contrast mechanisms. Serial blockface histology (SBH) systems using ICI modalities are then reported, followed by a review of some of their applications. These include validation studies and the creation of multimodal brain atlases at a micrometer resolution. The paper concludes with a perspective of future developments, calling for a consolidation of the SBH research and development efforts around the world. The goal would be to offer the neuroscience community a single standardized open-source SBH solution, including optical design, acquisition automation, reconstruction algorithms, and analysis pipelines. View Full-Text
Keywords: serial blockface histology; intrinsic optical contrast; brain imaging; neurophotonics serial blockface histology; intrinsic optical contrast; brain imaging; neurophotonics
Show Figures

Figure 1

MDPI and ACS Style

Lefebvre, J.; Delafontaine-Martel, P.; Lesage, F. A Review of Intrinsic Optical Imaging Serial Blockface Histology (ICI-SBH) for Whole Rodent Brain Imaging. Photonics 2019, 6, 66.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop