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Separations, Volume 8, Issue 1

2021 January - 9 articles

Cover Story: Therapeutic monoclonal antibodies (mAbs) are dominating the biopharmaceutical field due to their high specificity in the treatment of several diseases. Nevertheless, their high structural and functional complexity is challenging for mAb development, formulation, and quality control. Capillary electrophoresis has found many applications in mAb analyses at different mAb development steps, from clone selection to batch release. Its powerful features, such as efficient separation, miniaturization, automation, and coupling to mass spectrometry, allow a comprehensive characterization of mAbs at different levels (intact, top–down, middle–up/down, and bottom–up). View this paper.
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Articles (9)

  • Article
  • Open Access
4 Citations
3,902 Views
19 Pages

Nicotiana tabacum L. (NTL) is an important agricultural and economical crop. Its maturity is one of the key factors affecting its quality. Traditionally, maturity is discriminated visually by humans, which is subjective and empirical. In this study,...

  • Article
  • Open Access
30 Citations
5,827 Views
9 Pages

Antitumor Potential of Green Synthesized ZnONPs Using Root Extract of Withania somnifera against Human Breast Cancer Cell Line

  • Kollur Shiva Prasad,
  • Shashanka K Prasad,
  • Ravindra Veerapur,
  • Ghada Lamraoui,
  • Ashwini Prasad,
  • M. N. Nagendra Prasad,
  • Sandeep Kumar Singh,
  • Najat Marraiki,
  • Asad Syed and
  • Chandan Shivamallu

Herein we report the synthesis of zinc oxide nanoparticles (ZnONPs) using Withania somnifera root extract (WSE) as an effective chelating agent. The microscopic techniques viz., X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), tr...

  • Article
  • Open Access
27 Citations
13,945 Views
13 Pages

Dissolution Testing of Nicotine Release from OTDN Pouches: Product Characterization and Product-to-Product Comparison

  • Fadi Aldeek,
  • Nicholas McCutcheon,
  • Cameron Smith,
  • John H. Miller and
  • Timothy L. Danielson

In recent years, oral tobacco-derived nicotine (OTDN) pouches have emerged as a new oral tobacco product category. They are available in a variety of flavors and do not contain cut or ground tobacco leaf. The on!® nicotine pouches fall within thi...

  • Article
  • Open Access
17 Citations
5,008 Views
14 Pages

A highly specific, accurate, and simple RP-HPLC technique was developed for the real-time quantification of domperidone (DOMP) and lansoprazole (LANS) in commercial formulations. Chromatographic studies were performed using a Luna C8(2), 5 μm, 100...

  • Article
  • Open Access
7 Citations
4,018 Views
17 Pages

This study compares chloroform/methanol extraction and acidic methanol extraction of neutral compounds in absorbed lipid pottery residues from fourteen archaeological sherds. Previous studies have established that fatty acid extraction is more effect...

  • Feature Paper
  • Review
  • Open Access
41 Citations
22,890 Views
30 Pages

Therapeutic monoclonal antibodies (mAbs) are dominating the biopharmaceutical field due to the fact of their high specificity in the treatment of diverse diseases. Nevertheless, mAbs are very complex glycoproteins exhibiting several macro- and microh...

  • Review
  • Open Access
31 Citations
9,917 Views
19 Pages

Recent Advances in Microfluidics-Based Chromatography—A Mini Review

  • Bo Bao,
  • Zhichao Wang,
  • Dilantha Thushara,
  • Achini Liyanage,
  • Sanja Gunawardena,
  • Zaiyong Yang and
  • Shuangliang Zhao

Microfluidics-based liquid chromatography is based on the miniaturization of the different types of liquid chromatography (LC) systems (e.g., affinity, adsorption, size exclusion, ion exchange) on a microchip to perform on-chip separation of differen...

  • Article
  • Open Access
5 Citations
4,255 Views
14 Pages

A New Cytotoxic Dimeric Sesquiterpene Isolated from Inula racemosa Hook. f. (Root): In Vitro and In Silico Analyses

  • Rama Tyagi,
  • Perwez Alam,
  • Md. Tabish Rehman,
  • Mohamed Fahad AlAjmi,
  • Afzal Hussain,
  • Saima Amin,
  • Mohd. Mujeeb and
  • Showkat R. Mir

A new dimeric sesquiterpene named disesquicin (compound 1) was isolated from Inula racemosa roots by normal-phase MPLC (Medium Pressure Liquid Chromatography), and its structure was established by using extensive spectral analysis. Compound 1, when t...

  • Article
  • Open Access
14 Citations
4,892 Views
14 Pages

Extraction of Natural Pigments from Gardenia Jasminoides J.Ellis Fruit Pulp Using CO2-Expanded Liquids and Direct Sonication

  • Hiroki Sakai,
  • Kento Ono,
  • Shinichi Tokunaga,
  • Tanjina Sharmin,
  • Taku Michael Aida and
  • Kenji Mishima

In this work, a carbon dioxide-expanded liquid (CXL) extraction system was used with or without direct sonication for the extraction of highly polar natural pigments (crocin-1 and crocin-2) from Gardenia jasminoides Ellis fruit pulp. The effects of d...

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Separations - ISSN 2297-8739