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Article

Box-Behnken Assisted Validation and Optimization of an RP-HPLC Method for Simultaneous Determination of Domperidone and Lansoprazole

1
Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
2
Department of Pharmaceutical Technology, Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh 31400, Malaysia
3
School of Pharmacy, University of Nottingham Malaysia, Jalan Broga, Semenyih, Kajang, Selangor 43500, Malaysia
*
Author to whom correspondence should be addressed.
Separations 2021, 8(1), 5; https://doi.org/10.3390/separations8010005
Received: 29 November 2020 / Revised: 23 December 2020 / Accepted: 28 December 2020 / Published: 6 January 2021
A highly specific, accurate, and simple RP-HPLC technique was developed for the real-time quantification of domperidone (DOMP) and lansoprazole (LANS) in commercial formulations. Chromatographic studies were performed using a Luna C8(2), 5 μm, 100Å, column (250 × 4.6 mm, Phenomenex) with a mobile phase composed of acetonitrile/2 mM ammonium acetate (51:49 v/v), pH 6.7. The flow rate was 1 mL·min−1 with UV detection at 289 nm. Linearity was observed within the range of 4–36 µg·mL−1 for domperidone and 2–18 µg·mL−1 for lansoprazole. Method optimization was achieved using Box-Behnken design software, in which three key variables were examined, namely, the flow rate (A), the composition of the mobile phase (B), and the pH (C). The retention time (Y1 and Y3) and the peak area (Y2 and Y4) were taken as the response parameters. We observed that slight alterations in the mobile phase and the flow rate influenced the outcome, whereas the pH exerted no effect. Method validation featured various ICH parameters including linearity, limit of detection (LOD), accuracy, precision, ruggedness, robustness, stability, and system suitability. This method is potentially useful for the analysis of commercial formulations and laboratory preparations. View Full-Text
Keywords: lansoprazole; domperidone; method validation; Box–Behnken; chromatography lansoprazole; domperidone; method validation; Box–Behnken; chromatography
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MDPI and ACS Style

Afzal, M.; Muddassir, M.; Alarifi, A.; Ansari, M.T. Box-Behnken Assisted Validation and Optimization of an RP-HPLC Method for Simultaneous Determination of Domperidone and Lansoprazole. Separations 2021, 8, 5. https://doi.org/10.3390/separations8010005

AMA Style

Afzal M, Muddassir M, Alarifi A, Ansari MT. Box-Behnken Assisted Validation and Optimization of an RP-HPLC Method for Simultaneous Determination of Domperidone and Lansoprazole. Separations. 2021; 8(1):5. https://doi.org/10.3390/separations8010005

Chicago/Turabian Style

Afzal, Mohd, Mohd. Muddassir, Abdullah Alarifi, and Mohammed Tahir Ansari. 2021. "Box-Behnken Assisted Validation and Optimization of an RP-HPLC Method for Simultaneous Determination of Domperidone and Lansoprazole" Separations 8, no. 1: 5. https://doi.org/10.3390/separations8010005

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