Next Article in Journal
Development of Alternative Green Sample Preparation Techniques
Next Article in Special Issue
Role of Ionic Liquids in Composites in Analytical Sample Preparation
Previous Article in Journal
Current Trends in Simultaneous Determination of Co-Administered Drugs
Previous Article in Special Issue
Estimation of Solute-Stationary Phase and Solute-Mobile Phase Interactions in the Presence of Ionic Liquids
Open AccessArticle

Hydrophilic Liquid Chromatography versus Reversed-Phase Liquid Chromatography in the Absence and the Presence of 1-Hexyl-3-methylimidazolium Chloride for the Analysis of Basic Compounds

Departament de Química Analítica, Universitat de València, c/Dr. Moliner 50, 46100 Burjassot, Spain
*
Author to whom correspondence should be addressed.
Separations 2020, 7(2), 30; https://doi.org/10.3390/separations7020030
Received: 14 April 2020 / Revised: 23 May 2020 / Accepted: 27 May 2020 / Published: 29 May 2020
(This article belongs to the Special Issue Recent Advances on Ionic Liquid Uses in Separation Techniques)
In reversed-phase liquid chromatography (RPLC), positively charged basic compounds yield broad and asymmetric peaks, as a result of ionic interactions with free silanols that remain on conventional silica-based columns. Diverse solutions have been proposed to mask the silanophilic activity, which is translated to an improved peak shape. In this work, the chromatographic performance of hydrophilic interaction liquid chromatography (HILIC) was evaluated as an alternative to the addition of an ionic liquid (IL) to the aqueous-organic mobile phase used with RPLC columns, for the analysis of eight β-adrenoceptor antagonists. ILs change the behavior of RPLC stationary phases owing to adsorption on their surface. Meanwhile, in HILIC, a layer of adsorbed water is formed on the stationary phase surface. The association of cationic basic compounds with the adsorbed additive ions, hydrophilic partitioning on the HILIC columns, and other interactions, give rise to complex retention mechanisms. The chromatographic behavior was examined in terms of retention, elution strength, selectivity, peak shape and resolution, using acetonitrile-water mobile phases buffered at pH 3. Both chromatographic modes, RPLC with added IL and HILIC, proved to be a viable solution to the problem of poor peak shape for basic compounds. View Full-Text
Keywords: hydrophilic interaction liquid chromatography; reversed-phase liquid chromatography; ionic liquids; basic compounds; chromatographic behavior hydrophilic interaction liquid chromatography; reversed-phase liquid chromatography; ionic liquids; basic compounds; chromatographic behavior
Show Figures

Figure 1

MDPI and ACS Style

Peris-García, E.; Burgos-Gil, R.; García-Alvarez-Coque, M.C.; Ruiz-Angel, M.J. Hydrophilic Liquid Chromatography versus Reversed-Phase Liquid Chromatography in the Absence and the Presence of 1-Hexyl-3-methylimidazolium Chloride for the Analysis of Basic Compounds. Separations 2020, 7, 30.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop