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Open AccessCommunication

Phytochemical Composition and Enzyme Inhibition Studies of Buxus papillosa C.K. Schneid

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School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Malaysia
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Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore 54000, Pakistan
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Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Malaysia
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Department of Biology, Faculty of Science, Selcuk University, 42250 Konya, Turkey
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Department of Pharmacy, University ‘G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, Italy
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Liquid Chromatography Mass Spectrometry (LCMS) Platform, Monash University, Jalan Lagoon Selatan, Bandar Sunway 47500, Malaysia
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Tropical Medicine and Biology Multidisciplinary Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Malaysia
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Global Asia in the 21st Century (GA21) Multidisciplinary Research Platform, Monash University, Melbourne VIC 3800, Australia
*
Authors to whom correspondence should be addressed.
Processes 2020, 8(7), 757; https://doi.org/10.3390/pr8070757
Received: 23 April 2020 / Revised: 20 June 2020 / Accepted: 21 June 2020 / Published: 29 June 2020
(This article belongs to the Special Issue Production and Biomedical Applications of Bioactive Compounds)
The current research work is an endeavor to study the chemical profiling and enzyme-inhibition potential of different polarity solvent (n-hexane, dichloromethane—DCM and methanol—MeOH) extracts from the aerial and stem parts of Buxus papillosa C.K. Schneid. All the extracts were analyzed for HPLC-PDA phenolic quantification, while both (aerial and stem) DCM extracts were studied for UHPLC-MS phytochemical composition. The inhibitory activity against the clinically important enzymes having crucial role in different pathologies like skin diseases (tyrosinase), inflammatory problems (lipoxygenase—LOX) and diabetes mellitus (α-amylase) were studied using standard in vitro bioassays. The DCM extracts upon UHPLC-MS analysis conducted in both negative and positive ionization modes has led to the tentative identification of 52 important secondary metabolites. Most of these belonged to the alkaloid, flavonoid, phenolic and triterpenoid classes. The HPLC-PDA polyphenolic quantification identified the presence of 10 phenolic compounds. Catechin was present in significant amounts in aerial-MeOH (7.62 ± 0.45 μg/g extract) and aerial-DCM (2.39 ± 0.51-μg/g extract) extracts. Similarly, higher amounts of epicatechin (2.76 ± 0.32-μg/g extract) and p-hydroxybenzoic acid (1.06 ± 0.21 μg/g extract) were quantified in aerial-DCM and stem-MeOH extracts, respectively. Likewise, all the extracts exhibited moderate inhibition against all the tested enzymes. These findings explain the wide usage of this plant in folklore medicine and suggest that it could be further studied as an origin of novel bioactive phytocompounds and for the designing of new pharmaceuticals. View Full-Text
Keywords: Buxus papillosa; phenolic compounds; antioxidant; tyrosinase; amylase; lipoxygenase Buxus papillosa; phenolic compounds; antioxidant; tyrosinase; amylase; lipoxygenase
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Saleem, H.; Htar, T.T.; Naidu, R.; Zengin, G.; Locatelli, M.; Tartaglia, A.; Zainal Abidin, S.A.; Ahemad, N. Phytochemical Composition and Enzyme Inhibition Studies of Buxus papillosa C.K. Schneid. Processes 2020, 8, 757.

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