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Open AccessArticle

Optimizing the Extraction of Polysaccharides from Bletilla ochracea Schltr. Using Response Surface Methodology (RSM) and Evaluating their Antioxidant Activity

1
National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, China
2
Shanxi Institute of Medicine and Life Sciences, Taiyuan 030006, China
*
Author to whom correspondence should be addressed.
Processes 2020, 8(3), 341; https://doi.org/10.3390/pr8030341
Received: 24 February 2020 / Revised: 10 March 2020 / Accepted: 10 March 2020 / Published: 16 March 2020
(This article belongs to the Special Issue Extraction Optimization Processes of Antioxidants)
Bletilla ochracea Schltr. polysaccharides (BOP) have a similar structure to Bletilla striata (Thunb.) Reichb.f. (Orchidaceae) polysaccharides (BSP). Therefore, BOP can be considered as a substitute for BSP in the food, pharmaceuticals and cosmetics fields. To the best of our knowledge, little information is available regarding the optimization of extraction and antioxidant activity of BOP. In this study, response surface methodology (RSM) was firstly used for optimizing the extraction parameters of BOP. The results suggested that the optimal conditions included a temperature of 82 °C, a duration of 85 min and a liquid/material ratio of 30 mL/g. In these conditions, we received 26.45% ± 0.18% as the experimental yield. In addition, BOP exhibited strong concentration-dependent antioxidant abilities in vitro. The half-maximal effective concentration (EC50) values of BOP against 1,1-diphenyl-2-picrylhydrazyl (DPPH·), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonate) (ABTS+·), hydroxyl (·OH) and superoxide anion (·O2) radicals and ferrous ions (Fe2+) were determined as 692.16, 224.09, 542.22, 600.53 and 515.70 µg/mL, respectively. In conclusion, our results indicate that BOP can be a potential natural antioxidant, deserving further investigation. View Full-Text
Keywords: Bletilla ochracea Schltr. polysaccharide; response surface methodology (RSM); antioxidant activities Bletilla ochracea Schltr. polysaccharide; response surface methodology (RSM); antioxidant activities
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Wang, B.; Xu, Y.; Chen, L.; Zhao, G.; Mi, Z.; Lv, D.; Niu, J. Optimizing the Extraction of Polysaccharides from Bletilla ochracea Schltr. Using Response Surface Methodology (RSM) and Evaluating their Antioxidant Activity. Processes 2020, 8, 341.

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