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Open AccessArticle

Measurement and Modeling of Extra-Column Effects Due to Injection and Connections in Capillary Liquid Chromatography

1
Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA
2
Waters Corporation, Milford, MA 01757, USA
*
Author to whom correspondence should be addressed.
Current address: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
Current address: Seventh Sense Biosystems, Inc., Cambridge, MA 02141, USA
Academic Editor: Michael J. Dunphy
Chromatography 2015, 2(4), 669-690; https://doi.org/10.3390/chromatography2040669
Received: 24 August 2015 / Revised: 18 October 2015 / Accepted: 5 November 2015 / Published: 1 December 2015
(This article belongs to the Special Issue Advances in High Pressure Liquid Chromatography)
As column volumes continue to decrease, extra-column band broadening has become an increasingly important consideration when determining column performance. Combined contributions due to the injector and connecting tubing in a capillary LC system were measured and found to be larger than expected by Taylor-Aris theory. Variance from sigma-type and tau-type broadening was isolated from eluted peaks using the Foley-Dorsey Exponentially Modified Gaussian peak fitting model and confirmed with computational fluid dynamics. It was found that the tau-type contributions were the main cause for the excessive broadening because of poorly-swept volumes at the connection between the injector and tubing. To reduce tau-type contributions (and peak tailing), a timed pinch mode could be used for analyte injection. View Full-Text
Keywords: extra-column band broadening; injection; liquid chromatography; peak measurements extra-column band broadening; injection; liquid chromatography; peak measurements
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Grinias, J.P.; Bunner, B.; Gilar, M.; Jorgenson, J.W. Measurement and Modeling of Extra-Column Effects Due to Injection and Connections in Capillary Liquid Chromatography. Chromatography 2015, 2, 669-690.

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