Next Article in Journal
Insights into the Impact of Microbiota in the Treatment of NAFLD/NASH and Its Potential as a Biomarker for Prognosis and Diagnosis
Next Article in Special Issue
3D Printing for Soft Tissue Regeneration and Applications in Medicine
Previous Article in Journal
Novel Ex Vivo Model to Examine the Mechanism and Relationship of Esophageal Microbiota and Disease
Previous Article in Special Issue
Biocompatibility and Immune Response of a Newly Developed Volume-Stable Magnesium-Based Barrier Membrane in Combination with a PVD Coating for Guided Bone Regeneration (GBR)
Article

Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study

1
Department of Cranio-Maxillofacial Surgery, Inselspital, Bern University Hospital, Faculty of Medicine, University of Berne, CH-3010 Berne, Switzerland
2
Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, Niigata 951-8580, Japan
3
Department of Periodontology, School of Dental Medicine, University of Berne, CH-3010 Berne, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editor: Mike Barbeck
Biomedicines 2021, 9(2), 143; https://doi.org/10.3390/biomedicines9020143
Received: 18 January 2021 / Accepted: 27 January 2021 / Published: 2 February 2021
(This article belongs to the Special Issue Soft and Hard Tissue Regeneration)
The aim of this study was to evaluate the influence of additional hydroxyapatite (HA) in collagen-based matrices (CM) and membrane placement on bone formation in calvarial defects. Critical size defects in the calvaria of 16 New Zealand White Rabbits were randomly treated with CM or mineralized collagen-based matrices (mCM). Half of the sites were covered with a collagen membrane. Animals were euthanized after 12 weeks of healing. The samples were studied by micro-CT and histology. Newly formed lamellar bone was observed in all samples at the periphery of the defect. In the central areas, however, new bone composed of both woven and lamellar bone was embedded in the soft tissue. Samples treated with mCM showed more residual biomaterial and induced more small bony islands in the central areas of the defects than samples with CM. Nevertheless, a complete defect closure was not observed in any of the samples at 12 weeks. Membrane placement resulted in a decrease in bone density and height. Significant differences between the groups were revealed only between CM groups with and without membrane coverage for bone height in the central area of the defect. Neither mineralization of CM nor membrane placement improved the osteogenic capacity in this particular defect. Nevertheless, mineralisation influenced bone density without a membrane placement and bone volume underneath a membrane. CM may be used as a scaffold in bone regeneration procedures, without the need of a membrane coverage. Further preclinical studies are warrant to optimise the potential of mCM. View Full-Text
Keywords: animal experiment; critical size defect; collagen; hydroxyapatite; micro-CT; histological analysis animal experiment; critical size defect; collagen; hydroxyapatite; micro-CT; histological analysis
Show Figures

Figure 1

MDPI and ACS Style

Katagiri, H.; El Tawil, Y.; Lang, N.P.; Imber, J.-C.; Sculean, A.; Fujioka-Kobayashi, M.; Saulacic, N. Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study. Biomedicines 2021, 9, 143. https://doi.org/10.3390/biomedicines9020143

AMA Style

Katagiri H, El Tawil Y, Lang NP, Imber J-C, Sculean A, Fujioka-Kobayashi M, Saulacic N. Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study. Biomedicines. 2021; 9(2):143. https://doi.org/10.3390/biomedicines9020143

Chicago/Turabian Style

Katagiri, Hiroki, Yacine El Tawil, Niklaus P. Lang, Jean-Claude Imber, Anton Sculean, Masako Fujioka-Kobayashi, and Nikola Saulacic. 2021. "Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study" Biomedicines 9, no. 2: 143. https://doi.org/10.3390/biomedicines9020143

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop