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Evidence for Contamination of Silica Microparticles in Advanced Platelet-Rich Fibrin Matrices Prepared Using Silica-Coated Plastic Tubes

1
Private Practice, Hiroshima 732-0066, Japan
2
Private Practice, Kawasaki 213-0033, Kanagawa, Japan
3
Private Practice, Ohta 373-0808, Japan
4
Division of Anatomy and Cell Biology of the Hard Tissue, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
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Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, Japan
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Department of Oral and Maxillofacial Surgery, Matsumoto Dental University, Shiojiri 399-0781, Japan
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Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan
8
Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan
9
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
*
Author to whom correspondence should be addressed.
Biomedicines 2019, 7(2), 45; https://doi.org/10.3390/biomedicines7020045
Received: 20 May 2019 / Revised: 17 June 2019 / Accepted: 18 June 2019 / Published: 19 June 2019
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Abstract

Platelet-rich fibrin (PRF) therapy has been widely applied in regenerative dentistry, and PRF preparation has been optimized to efficiently form fibrin clots using plain glass tubes. Currently, a shortage of commercially available glass tubes has forced PRF users to utilize silica-coated plastic tubes. However, most plastic tubes are approved by regulatory authorities only for diagnostic use and remain to be approved for PRF therapy. To clarify this issue, we quantified silica microparticles incorporated into the PRF matrix. Blood samples were collected into three different brands of silica-containing plastic tubes and were immediately centrifuged following the protocol for advanced-PRF (A-PRF). Advanced-PRF-like matrices were examined using a scanning electron microscope (SEM), and silica microparticles were quantified using a spectrophotometer. Each brand used silica microparticles of specific size and appearance. Regardless of tube brands and individual donors, significant, but not accidental, levels of silica microparticles were found to be incorporated into the A-PRF-like matrix, which will be consequently incorporated into the implantation sites. Presently, from the increasing data for cytotoxicity of amorphous silica, we cannot exclude the possibility that such A-PRF-like matrices negatively influence tissue regeneration through induction of inflammation. Further investigation should be performed to clarify such potential risks. View Full-Text
Keywords: advanced platelet-rich fibrin; amorphous silica; coating; blood collection tube; health hazard advanced platelet-rich fibrin; amorphous silica; coating; blood collection tube; health hazard
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Tsujino, T.; Takahashi, A.; Yamaguchi, S.; Watanabe, T.; Isobe, K.; Kitamura, Y.; Tanaka, T.; Nakata, K.; Kawase, T. Evidence for Contamination of Silica Microparticles in Advanced Platelet-Rich Fibrin Matrices Prepared Using Silica-Coated Plastic Tubes. Biomedicines 2019, 7, 45.

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