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Biomedicines
Volume 13
Issue 5
10.3390/biomedicines13051218
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Human and Mouse Bone Marrow CD45+ Erythroid Cells Have a Constitutive Expression of Antibacterial Immune Response Signature Genes

by
Roman Perik-Zavodskii
1,
Olga Perik-Zavodskaia
1,
Julia Shevchenko
1,
Kirill Nazarov
1,
Anastasia Gizbrekht
1,
Saleh Alrhmoun
1,
Vera Denisova
2 and
Sergey Sennikov
1,*
1
Laboratory of Molecular Immunology, Research Institute of Fundamental and Clinical Immunology, Novosibirsk 630099, Russia
2
Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology, Novosibirsk 630099, Russia
*
Author to whom correspondence should be addressed.
Biomedicines 2025, 13(5), 1218; https://doi.org/10.3390/biomedicines13051218 (registering DOI)
Submission received: 14 April 2025 / Revised: 15 May 2025 / Accepted: 15 May 2025 / Published: 17 May 2025
(This article belongs to the Section Immunology and Immunotherapy)
Review Reports Versions Notes

Abstract

Introduction: Recent studies have shown that Erythroid progenitor cells exhibit a distinct immunosuppressive and immunoregulatory phenotype associated with the response to bacteria. Methods: The objective of this study was to comprehensively explore the traits of human bone marrow Erythroid cells through protein–protein interaction network analysis using cytokine secretion analysis, and single-cell immunoproteomic analysis using flow cytometry, as well as the re-analysis of publicly available human and mouse bone marrow Erythroid-cell transcriptomic data. Results: Our protein–protein interaction network analysis of human bone marrow Erythroid-cell protein-coding genes identified enrichment in the immune response to lipopolysaccharide, with Calprotectin and Cathepsin G being the main factors. We then mapped the Calprotectin to the CD45+ Erythroid cells of both humans and mice via the analysis of the publicly available scRNA-seq data. Additionally, we observed that human bone marrow Erythroid cells secrete cytokines and chemokines, such as IL-1b, IL-8, and IL-18, which are also mainly involved in the immune response to lipopolysaccharide. We also found that human and mouse bone marrow Erythroid-cell conditional media inhibit bacterial growth in vitro. Discussion: These findings suggest that both human and mouse bone marrow CD45+ Erythroid cells possess the potential to combat pathogenic microbes and thus play a role in innate antimicrobial immunity. Conclusions: CD45+ Erythroid cells are a potent immunoregulatory cell population in both humans and mice.
Keywords: erythroid cells; CD45+ erythroid cells; antibacterial immunity; lipopolysaccharide; LPS; calprotectin erythroid cells; CD45+ erythroid cells; antibacterial immunity; lipopolysaccharide; LPS; calprotectin

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MDPI and ACS Style

Perik-Zavodskii, R.; Perik-Zavodskaia, O.; Shevchenko, J.; Nazarov, K.; Gizbrekht, A.; Alrhmoun, S.; Denisova, V.; Sennikov, S. Human and Mouse Bone Marrow CD45+ Erythroid Cells Have a Constitutive Expression of Antibacterial Immune Response Signature Genes. Biomedicines 2025, 13, 1218. https://doi.org/10.3390/biomedicines13051218

AMA Style

Perik-Zavodskii R, Perik-Zavodskaia O, Shevchenko J, Nazarov K, Gizbrekht A, Alrhmoun S, Denisova V, Sennikov S. Human and Mouse Bone Marrow CD45+ Erythroid Cells Have a Constitutive Expression of Antibacterial Immune Response Signature Genes. Biomedicines. 2025; 13(5):1218. https://doi.org/10.3390/biomedicines13051218

Chicago/Turabian Style

Perik-Zavodskii, Roman, Olga Perik-Zavodskaia, Julia Shevchenko, Kirill Nazarov, Anastasia Gizbrekht, Saleh Alrhmoun, Vera Denisova, and Sergey Sennikov. 2025. "Human and Mouse Bone Marrow CD45+ Erythroid Cells Have a Constitutive Expression of Antibacterial Immune Response Signature Genes" Biomedicines 13, no. 5: 1218. https://doi.org/10.3390/biomedicines13051218

APA Style

Perik-Zavodskii, R., Perik-Zavodskaia, O., Shevchenko, J., Nazarov, K., Gizbrekht, A., Alrhmoun, S., Denisova, V., & Sennikov, S. (2025). Human and Mouse Bone Marrow CD45+ Erythroid Cells Have a Constitutive Expression of Antibacterial Immune Response Signature Genes. Biomedicines, 13(5), 1218. https://doi.org/10.3390/biomedicines13051218

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