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Article

A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation

1
Proteomics Core Facility and School of Life Sciences, The University of Technology Sydney, Ultimo, NSW 2007, Australia
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Neurotoxin Research Group, School of Life Sciences, The University of Technology Sydney, Ultimo, NSW 2007, Australia
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School of Life Sciences, The University of Technology Sydney, Ultimo, NSW 2007, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Jacek R. Wisniewski and Gunnar Dittmar
Proteomes 2021, 9(2), 24; https://doi.org/10.3390/proteomes9020024
Received: 19 March 2021 / Revised: 2 May 2021 / Accepted: 18 May 2021 / Published: 24 May 2021
Proteinopathies are protein misfolding diseases that have an underlying factor that affects the conformation of proteoforms. A factor hypothesised to play a role in these diseases is the incorporation of non-protein amino acids into proteins, with a key example being the therapeutic drug levodopa. The presence of levodopa as a protein constituent has been explored in several studies, but it has not been examined in a global proteomic manner. This paper provides a proof-of-concept method for enzymatically creating levodopa-containing proteins using the enzyme tyrosinase and provides spectral evidence of in vitro incorporation in addition to the induction of the unfolded protein response due to levodopa. View Full-Text
Keywords: misincorporation; post-translational modifications; PTM; Levodopa; L-DOPA misincorporation; post-translational modifications; PTM; Levodopa; L-DOPA
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MDPI and ACS Style

Steele, J.R.; Strange, N.; Rodgers, K.J.; Padula, M.P. A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation. Proteomes 2021, 9, 24. https://doi.org/10.3390/proteomes9020024

AMA Style

Steele JR, Strange N, Rodgers KJ, Padula MP. A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation. Proteomes. 2021; 9(2):24. https://doi.org/10.3390/proteomes9020024

Chicago/Turabian Style

Steele, Joel Ricky, Natalie Strange, Kenneth J. Rodgers, and Matthew P. Padula. 2021. "A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation" Proteomes 9, no. 2: 24. https://doi.org/10.3390/proteomes9020024

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