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Review

Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward

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Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, 60131 Ancona, Italy
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Laboratorio de Biotecnología, La Platina Research Station, Instituto de Investigaciones Agropecuarias, Santa Rosa, La Pintana, Santiago 11610, Chile
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Área de Genómica y Biotecnología, Grupo de Morfogénesis y Modificación Genética, IFAPA-Centro de Churriana, Cortijo de la Cruz s/n, 29140 Málaga, Spain
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USDA-ARS-Appalachian Fruit Research Station, 2217 Wiltshire Road, Kearneysville, WV 25430, USA
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Ralph Scorza LLC, Plant Breeding and Biotechnology Consulting Services, P.O. Box 1191, Shepherdstown, WV 25443, USA
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Mejora Genética de Cítricos, Instituto Murciano de Investigación y Desarrollo Agroalimentario (IMIDA), C/ Mayor s/n, 30150 Murcia, Spain
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Departamento de Mejora Vegetal, Grupo de Biotecnología de Frutales, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Espinardo, Murcia, Spain
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Departamento de Fruticultura Subtropical y Mediterránea, IHSM-UMA-CSIC, Avenida Dr. Wienberg, s/n. 29750 Algarrobo-Costa, Málaga, Spain
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Author to whom correspondence should be addressed.
Plants 2020, 9(8), 971; https://doi.org/10.3390/plants9080971
Received: 16 June 2020 / Revised: 28 July 2020 / Accepted: 29 July 2020 / Published: 31 July 2020
(This article belongs to the Special Issue Advances in Genetic Engineering Strategies for Fruit Crop Breeding)
Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation system. Despite the amount of time and the efforts invested, the lack of success has significantly limited the utility of peach as a model genetic system for trees, despite its relatively short generation time; small, high-quality genome; and well-studied genetic resources. Additionally, the absence of efficient genetic transformation protocols precludes the application of many biotechnological tools in peach breeding programs. In this review, we provide an overview of research on regeneration and genetic transformation in this species and summarize novel strategies and procedures aimed at producing transgenic peaches. Promising future approaches to develop a robust peach transformation system are discussed, focusing on the main bottlenecks to success including the low efficiency of A. tumefaciens-mediated transformation, the low level of correspondence between cells competent for transformation and those that have regenerative competence, and the high rate of chimerism in the few shoots that are produced following transformation. View Full-Text
Keywords: biotechnology; organogenesis; plant breeding; Rosaceae; somatic embryogenesis; stone fruits biotechnology; organogenesis; plant breeding; Rosaceae; somatic embryogenesis; stone fruits
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MDPI and ACS Style

Ricci, A.; Sabbadini, S.; Prieto, H.; Padilla, I.M.; Dardick, C.; Li, Z.; Scorza, R.; Limera, C.; Mezzetti, B.; Perez-Jimenez, M.; Burgos, L.; Petri, C. Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward. Plants 2020, 9, 971. https://doi.org/10.3390/plants9080971

AMA Style

Ricci A, Sabbadini S, Prieto H, Padilla IM, Dardick C, Li Z, Scorza R, Limera C, Mezzetti B, Perez-Jimenez M, Burgos L, Petri C. Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward. Plants. 2020; 9(8):971. https://doi.org/10.3390/plants9080971

Chicago/Turabian Style

Ricci, Angela, Silvia Sabbadini, Humberto Prieto, Isabel M. Padilla, Chris Dardick, Zhijian Li, Ralph Scorza, Cecilia Limera, Bruno Mezzetti, Margarita Perez-Jimenez, Lorenzo Burgos, and Cesar Petri. 2020. "Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward" Plants 9, no. 8: 971. https://doi.org/10.3390/plants9080971

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