Special Issue "Advances in Genetic Engineering Strategies for Fruit Crop Breeding"

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (31 July 2020).

Special Issue Editors

Prof. Dr. Bruno Mezzetti
E-Mail Website
Guest Editor
Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy
Interests: fruit plant breeding; biotechnology
Dr. Lorenzo Burgos
E-Mail Website
Guest Editor
CEBAS-CSIC – Departamento de Mejora Vegetal. Grupo de Biotecnologìa de Frutales, Murcia, Spain
Interests: fruit breeding; biotechnology; genetic transformation
Dr. Silvia Sabbadini
E-Mail Website
Guest Editor
Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy
Interests: Improving techniques for creating genetic variability in perennial fruit crops; In vitro regeneration approaches (organogenesis, somatic embryogenesis) to be applied for mutagenesis and NBTs applications; RNAi strategies (HIGS, SIGS); Cisgenesis/Intragenesis; Gene editing.
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Special Issue Information

Dear Colleagues,

Genetic engineering offers important opportunities for fruit crop genetic improvement of agronomic traits relevant for increasing sustainability of the cultivation systems and quality of the products used for fresh and processing industries. This potential presently looks enhanced by the availability of new breeding techniques (RNAi, cisgenesis, and gene editing). Furthermore, the application of RNAi technology in the rootstock offers the possibility to induce modifications in the scion without altering its genome. The main limiting factors for the application of these technologies in fruit crops are the development of efficient methods for direct and Agrobacterium-mediated genetic transformation, such as plant regeneration and selection of new modified events. In addition, the biosafety rules to be applied to new modified fruit plants are not yet fully explored. This Special Issue of Plants will highlight the new methods for genetic transformation/gene editing combined with modified plant regeneration and selection, the new type of gene/traits that can be improved, and the appropriate approaches for running the risk and benefits analyses of the new products.

Prof. Bruno Mezzetti
Dr. Lorenzo Burgos
Dr. Silvia Sabbadini
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fruit crops
  • genetic transformation
  • morphogenesis
  • selection
  • gene/traits of interest
  • field trials
  • risk and benefit analyses
  • consumer acceptance

Published Papers (2 papers)

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Review

Open AccessReview
Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward
Plants 2020, 9(8), 971; https://doi.org/10.3390/plants9080971 - 31 Jul 2020
Cited by 1 | Viewed by 1628
Abstract
Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation [...] Read more.
Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation system. Despite the amount of time and the efforts invested, the lack of success has significantly limited the utility of peach as a model genetic system for trees, despite its relatively short generation time; small, high-quality genome; and well-studied genetic resources. Additionally, the absence of efficient genetic transformation protocols precludes the application of many biotechnological tools in peach breeding programs. In this review, we provide an overview of research on regeneration and genetic transformation in this species and summarize novel strategies and procedures aimed at producing transgenic peaches. Promising future approaches to develop a robust peach transformation system are discussed, focusing on the main bottlenecks to success including the low efficiency of A. tumefaciens-mediated transformation, the low level of correspondence between cells competent for transformation and those that have regenerative competence, and the high rate of chimerism in the few shoots that are produced following transformation. Full article
(This article belongs to the Special Issue Advances in Genetic Engineering Strategies for Fruit Crop Breeding)
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Open AccessFeature PaperReview
Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus
Plants 2020, 9(8), 938; https://doi.org/10.3390/plants9080938 - 24 Jul 2020
Cited by 2 | Viewed by 1008
Abstract
Citrus is one of the most important fruit crops in the world. This review will discuss the recent findings related to citrus transformation and regeneration protocols of juvenile and adult explants. Despite the many advances that have been made in the last years [...] Read more.
Citrus is one of the most important fruit crops in the world. This review will discuss the recent findings related to citrus transformation and regeneration protocols of juvenile and adult explants. Despite the many advances that have been made in the last years (including the use of inducible promoters and site-specific recombination systems), transformation efficiency, and regeneration potential still represent a bottleneck in the application of the new breeding techniques in commercial citrus varieties. The influence of genotype, explant type, and other factors affecting the regeneration and transformation of the most used citrus varieties will be described, as well as some examples of how these processes can be applied to improve fruit quality and resistance to various pathogens and pests, including the potential of using genome editing in citrus. The availability of efficient regeneration and transformation protocols, together with the availability of the source of resistance, is made even more important in light of the fast diffusion of emerging diseases, such as Huanglongbing (HLB), which is seriously challenging citriculture worldwide. Full article
(This article belongs to the Special Issue Advances in Genetic Engineering Strategies for Fruit Crop Breeding)
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