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Open AccessArticle

Ethylenediaminetetraacetic Acid (EDTA) Mitigates the Toxic Effect of Excessive Copper Concentrations on Growth, Gaseous Exchange and Chloroplast Ultrastructure of Corchorus capsularis L. and Improves Copper Accumulation Capabilities

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MOA Key Laboratory of Crop Ecophysiology and Farming System Core in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
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Department of Environmental Sciences and Engineering, Government College University Allama Iqbal Road, Faisalabad 38000, Pakistan
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Department of Biological Sciences and Technology, China Medical University, Taichung 40402, Taiwan
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College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China
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Department of Botany, Government College University, Faisalabad 38000, Pakistan
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School of Environmental Studies, China university of Geosciences, Wuhan 430070, China
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Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
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Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
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Authors to whom correspondence should be addressed.
Plants 2020, 9(6), 756; https://doi.org/10.3390/plants9060756
Received: 7 April 2020 / Revised: 4 June 2020 / Accepted: 9 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Heavy Metal Stress in Plants)
Copper (Cu) is an important micronutrient for a plant’s normal growth and development. However, excess amount of Cu in the soil causes many severe problems in plants—which ultimately affect crop productivity and yield. Moreover, excess of Cu contents causes oxidative damage in the plant tissues by generating excess of reactive oxygen species (ROS). The present experiment was designed to investigate the phytoextraction potential of Cu, morpho-physiological features and biochemical reaction of jute (Corchorus capsularis L.) seedlings using ethylenediaminetetraacetic acid (EDTA) of 3 mM under different Cu levels (0 (control), 50 and 100 μM) in a hydroponic nutrient solution (Hoagland). Our results showed that elevated Cu rates (50 and 100 μM) in the nutrient solution significantly reduced plant height, fresh and dry biomass, total chlorophyll content and gaseous exchange attributes in C. capsularis seedlings. As the concentration of Cu in the medium increased (50 and 100 μM), the level of malondialdehyde (MDA) and oxidative stress in C. capsularis seedlings also increased, which could have been controlled by antioxidant activity in particular plant cells. In addition, rising Cu concentration in the nutrient solution also increased Cu uptake and accumulation in roots and leaves as well as affected the ultrastructure of chloroplast of C. capsularis seedlings. The addition of EDTA to the nutrient solution significantly alleviated Cu toxicity in C. capsularis seedlings, showing a significantly increase in plant growth and biomass. MDA contents was not significantly increased in EDTA-induced plants, suggesting that this treatment was helpful in capturing ROS and thereby reducing ROS in in C. capsularis seedlings. EDTA modification with Cu, although the bioaccumulation factor in roots and leaves and translocation factor for the leaves of C. capsularis seedlings has significantly increased. These results indicate that C. capsularis has considerable potential to cope with Cu stress and is capable of removing a large quantity of Cu from the Cu-contaminated soil while using EDTA is a useful strategy to increase plant growth and biomass with Cu absorption capabilities. View Full-Text
Keywords: fibrous crop; copper stress; chelating agent; phytoremediation; oxidative stress; ultrastructure of chloroplast fibrous crop; copper stress; chelating agent; phytoremediation; oxidative stress; ultrastructure of chloroplast
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Saleem, M.H.; Ali, S.; Kamran, M.; Iqbal, N.; Azeem, M.; Tariq Javed, M.; Ali, Q.; Zulqurnain Haider, M.; Irshad, S.; Rizwan, M.; Alkahtani, S.; M. Abdel-Daim, M. Ethylenediaminetetraacetic Acid (EDTA) Mitigates the Toxic Effect of Excessive Copper Concentrations on Growth, Gaseous Exchange and Chloroplast Ultrastructure of Corchorus capsularis L. and Improves Copper Accumulation Capabilities. Plants 2020, 9, 756.

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