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Open AccessArticle

High-Resolution Mapping of the Novel Early Leaf Senescence Gene Els2 in Common Wheat

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, China
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2020, 9(6), 698; https://doi.org/10.3390/plants9060698
Received: 1 April 2020 / Revised: 20 May 2020 / Accepted: 21 May 2020 / Published: 30 May 2020
(This article belongs to the Special Issue Plant Mutation Breeding)
Early leaf senescence negatively impacts the grain yield in wheat (Triticum aestivum L.). Induced mutants provide an important resource for mapping and cloning of genes for early leaf senescence. In our previous study, Els2, a single incomplete dominance gene, that caused early leaf senescence phenotype in the wheat mutant LF2099, had been mapped on the long arm of chromosome 2B. The objective of this study was to develop molecular markers tightly linked to the Els2 gene and construct a high-resolution map surrounding the Els2 gene. Three tightly linked single-nucleotide polymorphism (SNP) markers were obtained from the Illumina Wheat 90K iSelect SNP genotyping array and converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers. To saturate the Els2 region, the Axiom® Wheat 660K SNP array was used to screen bulked extreme phenotype DNA pools, and 9 KASP markers were developed. For fine mapping of the Els2 gene, these KASP markers and previously identified polymorphic markers were analyzed in a large F2 population of the LF2099 × Chinese Spring cross. The Els2 gene was located in a 0.24-cM genetic region flanked by the KASP markers AX-111643885 and AX-111128667, which corresponded to a physical interval of 1.61 Mb in the Chinese Spring chromosome 2BL containing 27 predicted genes with high confidence. The study laid a foundation for a map-based clone of the Els2 gene controlling the mutation phenotype and revealing the molecular regulatory mechanism of wheat leaf senescence. View Full-Text
Keywords: wheat (Triticum aestivum L.); early leaf senescence; mutant; single-nucleotide polymorphisms (SNPs); KASP markers; fine mapping wheat (Triticum aestivum L.); early leaf senescence; mutant; single-nucleotide polymorphisms (SNPs); KASP markers; fine mapping
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Wang, N.; Xie, Y.; Li, Y.; Wu, S.; Li, S.; Guo, Y.; Wang, C. High-Resolution Mapping of the Novel Early Leaf Senescence Gene Els2 in Common Wheat. Plants 2020, 9, 698.

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