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Open AccessArticle

Comparative Lipidomic Analysis Reveals Heat Stress Responses of Two Soybean Genotypes Differing in Temperature Sensitivity

1
Department of Plant and Environmental Sciences, Clemson University, Clemson, SC 29634, USA
2
Pee Dee Research and Education Center, Clemson University, Florence, SC 29506, USA
3
NCJ Diagnostics & DNA Technologies, Monmouth Junction, NJ 08852, USA
4
Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS 66506, USA
5
USDA, Agricultural Research Service, Crop Genetics Research Unit, Stoneville, MS 38776, USA
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(4), 457; https://doi.org/10.3390/plants9040457
Received: 8 March 2020 / Revised: 1 April 2020 / Accepted: 1 April 2020 / Published: 4 April 2020
(This article belongs to the Special Issue Function of Lipids in Plant Stress)
Heat-induced changes in lipidome and their influence on stress adaptation are not well-defined in plants. We investigated if lipid metabolic changes contribute to differences in heat stress responses in a heat-tolerant soybean genotype DS25-1 and a heat-susceptible soybean genotype DT97-4290. Both genotypes were grown at optimal temperatures (OT; 30/20 °C) for 15 days. Subsequently, half of the plants were exposed to heat stress (38/28 °C) for 11 days, and the rest were kept at OT. Leaf samples were collected for lipid and RNA extractions on the 9th and 11th days of stress, respectively. We observed a decline in the lipid unsaturation level due to a decrease in the polyunsaturated linolenic acid (18:3) content in DS25-1. When examined under OT conditions, DS25-1 and DT97-4290 showed no significant differences in the expression pattern of the Fatty Acid Desaturase (FAD) 2-1A, FAD2-2B, FAD2-2C, FAD3A genes. Under heat stress conditions, substantial reductions in the expression levels of the FAD3A and FAD3B genes, which convert 18:2 lipids to 18:3, were observed in DS25-1. Our results suggest that decrease in levels of lipids containing 18:3 acyl chains under heat stress in DS25-1 is a likely consequence of reduced FAD3A and FAD3B expression, and the decrease in 18:3 contributes to DS25-1′s maintenance of membrane functionality and heat tolerance. View Full-Text
Keywords: soybean; heat stress; lipidomics; lipid metabolic changes; lipid unsaturation; fatty acid desaturase soybean; heat stress; lipidomics; lipid metabolic changes; lipid unsaturation; fatty acid desaturase
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Narayanan, S.; Zoong-Lwe, Z.S.; Gandhi, N.; Welti, R.; Fallen, B.; Smith, J.R.; Rustgi, S. Comparative Lipidomic Analysis Reveals Heat Stress Responses of Two Soybean Genotypes Differing in Temperature Sensitivity. Plants 2020, 9, 457.

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