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Open AccessArticle

The Effect of Water-Soluble Polysaccharide from Jackfruit (Artocarpus heterophyllus Lam.) on Human Colon Carcinoma Cells Cultured In Vitro

1
Department of Industrial and Environmental Microbiology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland
2
Department of Virology and Immunology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland
3
Department of General Ophthalmology, Medical University, ul. Chmielna 1, 20-079 Lublin, Poland
4
Department of Genetics and Microbiology, Maria Curie-Skłodowska University, ul. Akademicka 19, 20-033 Lublin, Poland
5
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2020, 9(1), 103; https://doi.org/10.3390/plants9010103
Received: 2 December 2019 / Revised: 9 January 2020 / Accepted: 13 January 2020 / Published: 14 January 2020
(This article belongs to the Special Issue Plant Polysaccharides)
Various phytochemical studies have revealed that jackfruit (Artocarpus heterophyllus Lam.) is rich in bioactive compounds, including carotenoids, flavonoids, volatile acids, tannins, and lectins. The aim of the study was to analyze the biological activity of water-soluble polysaccharide (WSP) isolated from jackfruit and to assess its immunomodulatory, cytotoxic, and anti-oxidative effects on human colon carcinoma cells in vitro. The neutral red (NR) uptake assay revealed no toxic influence of the polymer on the viability of tumor cells (HT29 and SW620). After 24 h and 48 h of incubation, the cellular viability was not lower than 94%. The metabolic activity of the cells (MTT) at the compound concentration of 250 µg/mL was higher than 92% in comparison to the control. WSP (250 µg/mL) exerted no significant effect on the morphology of the cells was determined by May-Grünwald-Giemsa staining. WSP changed nitric oxide (NOx) production by the tumor cells depending on the time of incubation and prior 2-h stimulation of the cells with E. coli 0111:B4 LPS. It significantly stimulated IL-1β production by the tumor cells. The IL-6 level increased but that of IL-10 decreased by a WSP concentration-dependent manner. No such effect was detected in SW620. The WSP had antioxidant properties. In conclusion, water-soluble polysaccharide isolated from A. heterophyllus exhibits significant biological activity towards many types of both normal and cancerous cells. Therefore, it may be considered as a useful agent in the protection of human health or in functional and dietary nutrition.
Keywords: Artocarpus heterophyllus; water-soluble polysaccharide; cytotoxicity; cytokines; nitric oxide; human colon tumor cells Artocarpus heterophyllus; water-soluble polysaccharide; cytotoxicity; cytokines; nitric oxide; human colon tumor cells
MDPI and ACS Style

Wiater, A.; Paduch, R.; Trojnar, S.; Choma, A.; Pleszczyńska, M.; Adamczyk, P.; Pięt, M.; Próchniak, K.; Szczodrak, J.; Strawa, J.; Tomczyk, M. The Effect of Water-Soluble Polysaccharide from Jackfruit (Artocarpus heterophyllus Lam.) on Human Colon Carcinoma Cells Cultured In Vitro. Plants 2020, 9, 103.

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