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Plants 2019, 8(2), 36; https://doi.org/10.3390/plants8020036

The Power of Genomic in situ Hybridization (GISH) in Interspecific Breeding of Bulb Onion (Allium cepa L.) Resistant to Downy Mildew (Peronospora destructor [Berk.] Casp.)

Center of Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, 49, Timiryazevskaya Str., 127550 Moscow, Russia
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Received: 11 December 2018 / Revised: 28 January 2019 / Accepted: 29 January 2019 / Published: 4 February 2019
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

We exploited the advantages of genomic in situ hybridization (GISH) to monitor the introgression process at the chromosome level using a simple and robust molecular marker in the interspecific breeding of bulb onion (Allium cepa L.) that is resistant to downy mildew. Downy mildew (Peronospora destructor [Berk.] Casp.) is the most destructive fungal disease for bulb onions. With the application of genomic in situ hybridization (GISH) and previously developed DMR1 marker, homozygous introgression lines that are resistant to downy mildew were successfully produced in a rather short breeding time. Considering that the bulb onion is a biennial plant, it took seven years from the F1 hybrid production to the creation of S2BC2 homozygous lines that are resistant to downy mildew. Using GISH, it was shown that three progeny plants of S2BC2 possessed an A. roylei homozygous fragment in the distal region of the long arm of chromosomes 3 in an A. cepa genetic background. Previously, it was hypothesized that a lethal gene(s) was linked to the downy mildew resistance gene. With the molecular cytogenetic approach, we physically mapped more precisely the lethal gene(s) using the homozygous introgression lines that differed in the size of the A. roylei fragments on chromosome 3. View Full-Text
Keywords: genomic in situ hybridization (GISH); Allium cepa; A. roylei; downy mildew resistance; lethal factor; DMR1 marker genomic in situ hybridization (GISH); Allium cepa; A. roylei; downy mildew resistance; lethal factor; DMR1 marker
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Khrustaleva, L.; Mardini, M.; Kudryavtseva, N.; Alizhanova, R.; Romanov, D.; Sokolov, P.; Monakhos, G. The Power of Genomic in situ Hybridization (GISH) in Interspecific Breeding of Bulb Onion (Allium cepa L.) Resistant to Downy Mildew (Peronospora destructor [Berk.] Casp.). Plants 2019, 8, 36.

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