Next Article in Journal
Identification of Soil Properties Associated with the Incidence of Banana Wilt Using Supervised Methods
Next Article in Special Issue
Development of a Highly Efficient Shoot Organogenesis System for an Ornamental Aeschynanthus pulcher (Blume) G. Don Using Leaves as Explants
Previous Article in Journal
Metal(loid)s in Common Medicinal Plants in a Uranium Mining-Impacted Area in Northwestern New Mexico, USA
 
 
Article

The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)

1
Department of Field Crops, Faculty of Agriculture, Necmettin Erbakan University, 42310 Konya, Türkiye
2
Department of Field Crops, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Türkiye
3
Department of Biology, Faculty of Science, Cankiri Karatekin University, 18200 Çankırı, Türkiye
4
Hemp Research Institute, Ondokuz Mayıs University, 55200 Samsun, Türkiye
5
Health Services Vocational School, Erzincan Binali Yıldırım University, 24100 Erzincan, Türkiye
6
Department of Biology, Faculty of Science, Ankara University, 06100 Ankara, Türkiye
7
Botany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014 Helsinki, Finland
8
Institute of Advanced Studies Kőszeg (iASK), P.O. Box 4, H-9731 Kőszeg, Hungary
*
Authors to whom correspondence should be addressed.
Academic Editor: Jonny Everson Scherwinski-Pereira
Plants 2022, 11(15), 2071; https://doi.org/10.3390/plants11152071
Received: 7 July 2022 / Revised: 22 July 2022 / Accepted: 6 August 2022 / Published: 8 August 2022
(This article belongs to the Special Issue Somatic Embryogenesis and Plant Regeneration)
Mammalian sex hormones are steroid-structured compounds that support the growth and development of plants at low concentrations. Since they affect the physiological processes in plants, it has been thought that mammalian sex hormones may cause modifications to plant genomes and epigenetics. This study aims to determine whether different mammalian sex hormones (17 β-estradiol, estrogen, progesterone, and testosterone) in several concentrations (0, 10−4, 10−6, and 10−8 mM) affect genetic or epigenetic levels in bean plants, using in vitro tissue cultures from plumule explants. We investigated levels of DNA damage, changes in DNA methylation and DNA stability in common bean exposed to mammalian sex hormones (MSH) using inter-primer binding site (iPBS) and Coupled Restriction Enzyme Digestion-iPBS (CRED-iPBS) assays, respectively. The highest rate of polymorphism in iPBS profiles was observed when 10−4 mM of estrogen (52.2%) hormone was administered. This finding indicates that genetic stability is reduced. In the CRED-iPBS profile, which reveals the methylation level associated with the DNA cytosine nucleotide, 10−4 mM of estrogen hormone exhibited the highest hypermethylation value. Polymorphism was observed in all hormone administrations compared to the control (without hormone), and it was determined that genomic stability was decreased at high concentrations. Taken together, the results indicate that 17 β-estradiol, estrogen, progesterone, and testosterone in bean plants affect genomic instability and cause epigenetic modifications, which is an important control mechanism in gene expression. View Full-Text
Keywords: CRED-iPBS; epigenetics; genotoxic; in vitro CRED-iPBS; epigenetics; genotoxic; in vitro
Show Figures

Figure 1

MDPI and ACS Style

Türkoğlu, A.; Haliloğlu, K.; Balpinar, Ö.; Öztürk, H.I.; Özkan, G.; Poczai, P. The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.). Plants 2022, 11, 2071. https://doi.org/10.3390/plants11152071

AMA Style

Türkoğlu A, Haliloğlu K, Balpinar Ö, Öztürk HI, Özkan G, Poczai P. The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.). Plants. 2022; 11(15):2071. https://doi.org/10.3390/plants11152071

Chicago/Turabian Style

Türkoğlu, Aras, Kamil Haliloğlu, Özge Balpinar, Halil Ibrahim Öztürk, Güller Özkan, and Peter Poczai. 2022. "The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)" Plants 11, no. 15: 2071. https://doi.org/10.3390/plants11152071

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop