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Article

Novel Mutation in the Acetohydroxyacid Synthase (AHAS), Gene Confers Imidazolinone Resistance in Chickpea Cicer arietinum L. Plants

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Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, P.O. Box 15159, HaMaccabim Road 68, Rishon LeZion 7528809, Israel
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Newe Ya’ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay 3009503, Israel
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Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
*
Authors to whom correspondence should be addressed.
Academic Editors: Hudson Takano and Hugh J. Beckie
Plants 2021, 10(12), 2791; https://doi.org/10.3390/plants10122791
Received: 11 October 2021 / Revised: 7 December 2021 / Accepted: 10 December 2021 / Published: 16 December 2021
(This article belongs to the Special Issue Herbicide Mechanisms of Action and Resistance)
Chickpea (Cicer arietinum L.) is an important crop in crop-rotation management in Israel. Imidazolinone herbicides have a wide spectrum of weed control, but chickpea plants are sensitive to acetohydroxyacid synthase (AHAS; also known as acetolactate synthase [ALS]) inhibitors. Using the chemical mutagen ethyl methanesulfonate (EMS), we developed a chickpea line (M2033) that is resistant to imidazolinone herbicides. A point mutation was detected in one of the two genes encoding the AHAS catalytic subunit of M2033. The transition of threonine to isoleucine at position 192 (203 according to Arabidopsis) conferred resistance of M2033 to imidazolinones, but not to other groups of AHAS inhibitors. The role of this substitution in the resistance of line M2033 was proven by genetic transformation of tobacco plants. This resistance showed a single-gene semidominant inheritance pattern. Conclusion: A novel mutation, T192I (T203I according to Arabidopsis), providing resistance to IMI herbicides but not to other groups of AHAS inhibitors, is described in the AHAS1 protein of EMS-mutagenized chickpea line M2033. View Full-Text
Keywords: imidazolinone herbicide; herbicide resistance; acetohydroxyacid synthase (AHAS); chickpea; Cicer arietinum L.; mutagenesis imidazolinone herbicide; herbicide resistance; acetohydroxyacid synthase (AHAS); chickpea; Cicer arietinum L.; mutagenesis
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MDPI and ACS Style

Galili, S.; Hershenhorn, J.; Edelman, M.; Sobolev, V.; Smirnov, E.; Amir-Segev, O.; Bellalou, A.; Dor, E. Novel Mutation in the Acetohydroxyacid Synthase (AHAS), Gene Confers Imidazolinone Resistance in Chickpea Cicer arietinum L. Plants. Plants 2021, 10, 2791. https://doi.org/10.3390/plants10122791

AMA Style

Galili S, Hershenhorn J, Edelman M, Sobolev V, Smirnov E, Amir-Segev O, Bellalou A, Dor E. Novel Mutation in the Acetohydroxyacid Synthase (AHAS), Gene Confers Imidazolinone Resistance in Chickpea Cicer arietinum L. Plants. Plants. 2021; 10(12):2791. https://doi.org/10.3390/plants10122791

Chicago/Turabian Style

Galili, Shmuel, Joseph Hershenhorn, Marvin Edelman, Vladimir Sobolev, Evgeny Smirnov, Orit Amir-Segev, Aharon Bellalou, and Evgenia Dor. 2021. "Novel Mutation in the Acetohydroxyacid Synthase (AHAS), Gene Confers Imidazolinone Resistance in Chickpea Cicer arietinum L. Plants" Plants 10, no. 12: 2791. https://doi.org/10.3390/plants10122791

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