There was an error in the original publication [1]. Table S1 was an incomplete version, and Videos S1–S4, along with Tables S2–S8, were mistakenly omitted from the Supplementary Materials. The corrected Tables and Videos appear below:
Supplementary Video S1:
Supplementary Video S2:
Supplementary Video S3:
Supplementary Video S4:
Table S1. Raw measurement data for mouse molar, total jaw, and retromolar lengths. (https://drive.google.com/file/d/1pFWmqRkFrHfzMSoS_5wRE-cuTsoUUAiQ/view), and Tables S2–S8 appear below.
A correction has been made to the Supplementary Materials, and they are available online at https://www.mdpi.com/2221-3759/9/1/8/s1. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Table S2.
Descriptions of measurements used for embryonic and perinatal/postnatal specimens ranging from embryonic day (E) 10 to postnatal day (P) 32.
Table S2.
Descriptions of measurements used for embryonic and perinatal/postnatal specimens ranging from embryonic day (E) 10 to postnatal day (P) 32.
| Measurement Ages | Measurement Parameters | Notes |
|---|---|---|
| E10-18: Lower jaw length (Figure 4) P0-P32: Lower jaw length (Figure 5) | Anterior-most midline point on (mineralized tissue1 of) the mandible, measured to the; Anterior-most point of subcondylar incisive Most anterior-superior midline point of the alveolar rim between the incisors, to the; Anterior-most point of subcondylar incisive |
|
| E10-18: Upper jaw length (Figure 4) P0-P32: Upper jaw length (Figure 5) | Anterior-most point of the maxilla3, measured to the; Posterior-most point of the maxilla2. Most anterior-inferior point of the premaxilla between the incisors, to the; Posterior-most point of the maxilla2. | |
| E10-P32: Molar length (Figure 5) | Anterior-most point of molar organ or mineralizing/ed crown, measured to the; Posterior-most point of the same molar organ or mineralizing/ed crown4. | |
| E12-P32: Retromolar length5 (Figures 4 and 5) | Posterior landmark of latest-forming molar organ/crown, measured to the; Posterior-most point of the mandibular foramen2,6 of the mandible. |
Table S3.
From stages/ages E14 to P32, total upper jaw length in micrometres (µm), and the percent (%) of total upper jaw length occupied by upper first (M1), second (M2) and third (M3) molar mesiodistal crypt or crown lengths, as well as retromolar jaw length (3–35%) and remaining (i.e., non-molar, non retromolar) jaw length (~40–55%) for the diastema and incisor region. For every stage/age: there is space distal to the last-initiated molar; no notable increase in retromolar (RM) jaw length prior to M3 onset, and; M1 > M2 > M3 although M1 is longer and M3 shorter compared to their lower counterparts) Measurements are from the upper left side only, averaged for each stage/age (see Table 5 for full data used in this table).
Table S3.
From stages/ages E14 to P32, total upper jaw length in micrometres (µm), and the percent (%) of total upper jaw length occupied by upper first (M1), second (M2) and third (M3) molar mesiodistal crypt or crown lengths, as well as retromolar jaw length (3–35%) and remaining (i.e., non-molar, non retromolar) jaw length (~40–55%) for the diastema and incisor region. For every stage/age: there is space distal to the last-initiated molar; no notable increase in retromolar (RM) jaw length prior to M3 onset, and; M1 > M2 > M3 although M1 is longer and M3 shorter compared to their lower counterparts) Measurements are from the upper left side only, averaged for each stage/age (see Table 5 for full data used in this table).
| Stage/Age | Upper TJL (µm) | MD Molar Length as % of Upper TJL | % of Upper TJL | |||
|---|---|---|---|---|---|---|
| M1 | M2 | M3 | RML | Remaining | ||
| E12 | 1117.42 | 32.4 | - | - | - | - |
| E13 * | 3646.20 | 51.7 | - | - | 2.34 | 46.0 |
| E14 | 1809.08 | 36.3 | 21.5 | - | - | 42.2 |
| E15 | 2436.74 | 30.8 | 17.7 | - | 4.96 | 46.5 |
| E16 | 2519.10 | 34.4 | 12.8 | - | - | 52.8 |
| E17 | 3643.86 | 24.0 | 15.9 | - | 5.03 | 55.1 |
| E18 | 3663.62 | 33.3 | 14.0 | - | 9.60 | 43.1 |
| P0 | 4109.65 | 29.1 | 16.1 | - | 6.28 | 48.5 |
| P3 | 4590.23 | 31.3 | 18.0 | - | 5.02 | 45.7 |
| P6 | 3715.06 | 31.2 | 16.6 | - | 5.82 | 46.4 |
| P8 | 4158.42 | 28.6 | 16.3 | - | 10.86 | 44.2 |
| P12 | 4712.02 | 26.1 | 13.6 | 5.9 | 5.50 | 48.9 |
| P15 | 4992.52 | 25.0 | 14.5 | 8.1 | 3.98 | 48.4 |
| P18 | 5109.28 | 24.4 | 14.6 | 8.4 | 4.21 | 48.4 |
| P21 | 8102.80 | 21.4 | 13.0 | 8.1 | 5.14 | 52.4 |
| P23 | 8646.60 | 21.0 | 12.8 | 7.6 | 6.40 | 52.2 |
| P26 | 8355.17 | 21.8 | 13.1 | 7.9 | 5.38 | 51.8 |
| P28 | 9071.21 | 19.6 | 12.0 | 7.3 | 5.31 | 55.8 |
| P30 | 8328.67 | 19.5 | 12.5 | 7.6 | 5.12 | 55.3 |
| P32 | 9045.49 | 19.0 | 11.6 | 7.3 | 7.98 | 54.1 |
“-” indicates that at least one molar was not yet forming and thus no value could be measured for that stage/age group. * As noted in the main text and tables, E13 is inexplicably large and we use these data for proportions only. Abbreviations: MD, mesiodistal; RML, retromolar length; TJL, total jaw length.
Table S4.
From stages/ages E14 to P32, total lower jaw length in micrometres (µm), and the percent (%) of total lower jaw length occupied by lower first (M1), second (M2) and third (M3) molar mesiodistal crypt or crown lengths, as well as retromolar jaw length (~15–30%) and remaining (i.e., non-molar, non retromolar) jaw length (~35–50%) for the diastema and incisor region. For every stage/age: there is space distal to the last-initiated molar (i.e., no shortage of space); no notable increase in retromolar jaw length before M3 onset (P12); also, the entire molar row length is ~30–40% of the total lower jaw length, and M1 > M2 > M3. Measurements are from the lower left side only, averaged for each stage/age (see Table 5 for full data used in this table).
Table S4.
From stages/ages E14 to P32, total lower jaw length in micrometres (µm), and the percent (%) of total lower jaw length occupied by lower first (M1), second (M2) and third (M3) molar mesiodistal crypt or crown lengths, as well as retromolar jaw length (~15–30%) and remaining (i.e., non-molar, non retromolar) jaw length (~35–50%) for the diastema and incisor region. For every stage/age: there is space distal to the last-initiated molar (i.e., no shortage of space); no notable increase in retromolar jaw length before M3 onset (P12); also, the entire molar row length is ~30–40% of the total lower jaw length, and M1 > M2 > M3. Measurements are from the lower left side only, averaged for each stage/age (see Table 5 for full data used in this table).
| Stage/Age | Lower TJL (µm) | MD Molar Length as % of Lower TJL | % of Lower TJL | |||
|---|---|---|---|---|---|---|
| M1 | M2 | M3 | RML | Remaining | ||
| E12 | 1070.77 | 39.65 | - | - | ||
| E13 * | 4799.40 | 20.8 | - | - | ||
| E14 | 2304.25 | 25.5 | 10.9 | - | 9.81 | 53.79 |
| E15 | 3493.51 | 23.1 | 6.4 | - | 18.64 | 51.86 |
| E16 | 3612.25 | 24.5 | 6.3 | - | 14.60 | 54.60 |
| E17 | 3576.85 | 25.9 | 13.7 | - | 11.41 | 48.99 |
| E18 | 3827.62 | 26.8 | 14.1 | - | 14.24 | 44.86 |
| P0 | 5057.14 | 24.3 | 10.4 | - | 14.63 | 50.67 |
| P3 | 5171.79 | 25.1 | 16.7 | - | 14.02 | 44.18 |
| P6 | 4431.37 | 21.2 | 14.0 | - | 17.83 | 53.03 |
| P8 | 4721.25 | 21.4 | 14.8 | - | 22.77 | 41.03 |
| P12 | 5102.26 | 20.0 | 14.0 | 7.6 | 18.94 | 39.46 |
| P15 | 5570.41 | 18.5 | 13.3 | 8.7 | 22.59 | 36.91 |
| P18 | 5603.85 | 18.0 | 12.2 | 8.8 | 22.15 | 38.85 |
| P21 | 8447.00 | 17.3 | 11.8 | 9.8 | 22.27 | 38.83 |
| P23 | 8955.31 | 16.4 | 11.8 | 9.5 | 23.87 | 38.43 |
| P26 | 9137.99 | 16.2 | 10.5 | 10.0 | 24.3 | 39.00 |
| P28 | 9472.03 | 15.0 | 10.7 | 8.7 | 25.03 | 40.57 |
| P30 | 8688.78 | 16.6 | 11.2 | 9.5 | 25.31 | 37.40 |
| P32 | 9292.92 | 15.7 | 10.5 | 9.3 | 27.76 | 36.75 |
“-” indicates that at least one molar was not yet forming and could not be measured. * As noted in the main text and tables, E13 is inexplicably large and we use these data for proportions only. Abbreviations: MD, mesiodistal; RML, retromolar length; TJL, total jaw length.
Table S5.
From E10 to P3, linear regression model for day (independent variable) versus molar length (M1, M2, M3) showing that stage/age accurately predicts molar and jaw lengths but not retromolar lengths before and shortly after birth. M3 is not included as no data for this variable exists at these young stages/ages.
Table S5.
From E10 to P3, linear regression model for day (independent variable) versus molar length (M1, M2, M3) showing that stage/age accurately predicts molar and jaw lengths but not retromolar lengths before and shortly after birth. M3 is not included as no data for this variable exists at these young stages/ages.
| Dependent Variable | Jaw Type | Estimate (SE) | p-Value |
|---|---|---|---|
| M1 | Md | 92.77 (9.92) | <0.0001 |
| Mx | 106.03 (9.64) | <0.0001 | |
| M2 | Md | 76.04 (11.70) | 0.0013 |
| Mx | 55.80 (14.79) | 0.0130 | |
| Total jaw length | Md | 440.88 (42.04) | <0.0001 |
| Mx | 379.70 (37.06) | <0.0001 | |
| Retromolar length | Md | 56.73 (17.77) | 0.0242 * |
| Mx | 10.91 (19.60) | 0.6167 * |
All quadrants are left side. *, not significant; Md, mandible; Mx, maxilla; SE, standard error.
Table S6.
From P6 to P32, linear regression model for day (independent variable) versus molar length (M1, M2, M3), total jaw length, and retromolar length, showing that stage/age accurately predicts all measurements with the exception of upper retromolar length.
Table S6.
From P6 to P32, linear regression model for day (independent variable) versus molar length (M1, M2, M3), total jaw length, and retromolar length, showing that stage/age accurately predicts all measurements with the exception of upper retromolar length.
| Dependent Variable | Jaw Type | Estimate (SE) | p-Value |
|---|---|---|---|
| M1 | Md | 23.90 (4.17) | 0.0003 |
| Mx | 23.89 (5.25) | 0.0004 | |
| M2 | Md | 16.02 (2.83) | 0.0003 |
| Mx | 21.86 (3.23) | <0.0001 | |
| M3 | Md | 25.01 (5.63) | 0.0030 |
| Mx | 17.65 (4.65) | 0.0055 | |
| Total jaw length | Md | 221.54 (26.71) | <0.0001 |
| Mx | 240.02 (31.03) | <0.0001 | |
| Retromolar length | Md | 69.03 (7.49) | <0.0001 |
| Mx | 14.31 (4.97) | 0.0182 * |
All quadrants are left side. *, not significant; Md, mandible; Mx, maxilla; SE, standard error.
Table S7.
Linear regression model for total jaw length (independent variable) versus molar length (M1, M2, M3) showing that jaw length accurately predicts molar length.
Table S7.
Linear regression model for total jaw length (independent variable) versus molar length (M1, M2, M3) showing that jaw length accurately predicts molar length.
| Dependent Variable | Jaw Type | Estimate (SE) | p-Value |
|---|---|---|---|
| M1 | Md | 0.11 (0.01) | <0.0001 |
| Mx | 0.15 (0.01) | 0.0001 | |
| M2 | Md | 0.11 (0.01) | <0.0001 |
| Mx | 0.10 (0.01) | <0.0001 | |
| M3 | Md | 0.11 (0.01) | <0.0001 |
| Mx | 0.07 (0.01) | 0.0001 |
All quadrants are left side. Md, mandible; Mx, maxilla; SE, standard error.
Table S8.
Linear regression model for retromolar length (independent variable) versus molar length (M1, M2, M3) showing that, in general, retromolar length accurately predicts molar length, particularly in the mandible.
Table S8.
Linear regression model for retromolar length (independent variable) versus molar length (M1, M2, M3) showing that, in general, retromolar length accurately predicts molar length, particularly in the mandible.
| Dependent Variable | Jaw Type | Estimate (SE) | p-Value |
|---|---|---|---|
| M1 | Md | 0.31 (0.04) | <0.0001 |
| Mx | 1.53 (0.32) | 0.0003 | |
| M2 | Md | 0.33 (0.04) | <0.0001 |
| Mx | 1.07 (0.23) | 0.0003 | |
| M3 | Md | 0.34 (0.03) | <0.0001 |
| Mx | 0.57 (0.19) | 0.02 * |
All quadrants are left side. *, not significant; Md, mandible; Mx, maxilla; SE, standard error.
Reference
- Ko, D.; Kelly, T.; Thompson, L.; Uppal, J.K.; Rostampour, N.; Webb, M.A.; Zhu, N.; Belev, G.; Mondal, P.; Cooper, D.M.L.; et al. Timing of Mouse Molar Formation Is Independent of Jaw Length Including Retromolar Space. J. Dev. Bio. 2021, 9, 8. [Google Scholar] [CrossRef]
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