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Open AccessArticle

A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model

1
Department of Otorhinolaryngology, Head and Neck Surgery, Kansai Medical University, Hirakata 573-1010, Japan
2
Allergy Center, Kansai Medical University Hospital, Hirakata 573-1010, Japan
3
Central Research Laboratory, Kansai Medical University, Hirakata 573-1010, Japan
4
Department of Radiology, Kansai Medical University, Hirakata 573-1010, Japan
5
Department of Information Systems, Faculty of Information Science and Technology, Osaka Institute of Technology, Hirakata 573-0196, Japan
6
Nakamura ENT Clinic, Sakai 591-8025, Japan
7
University of Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011-EGID, F-5900 Lille, France
*
Author to whom correspondence should be addressed.
Authors share first authorship.
Biomolecules 2019, 9(7), 252; https://doi.org/10.3390/biom9070252
Received: 13 May 2019 / Revised: 18 June 2019 / Accepted: 25 June 2019 / Published: 27 June 2019
(This article belongs to the Special Issue Eosinophilic Inflammation)
Airway hyperresponsiveness (AHR) has been proposed as a feature of pathogenesis of eosinophilic upper airway inflammation such as allergic rhinitis (AR). The measurement system for upper AHR (UAHR) in rodents is poorly developed, although measurements of nasal resistance have been reported. Here we assessed UAHR by direct measurement of swelling of the nasal mucosa induced by intranasal methacholine (MCh) using micro-computed tomography (micro-CT). Micro-CT analysis was performed in both naïve and ovalbumin-induced AR mice following intranasal administration of MCh. The nasal cavity was segmented into two-dimensional horizontal and axial planes, and the data for nasal mucosa were acquired for the region of interest threshold. Then, a ratio between the nasal mucosa area and nasal cavity area was calculated as nasal mucosa index. Using our novel method, nasal cavity structure was clearly identified on micro-CT, and dose-dependent increased swelling of the nasal mucosa was observed upon MCh treatment. Moreover, the nasal mucosa index was significantly increased in AR mice compared to controls following MCh treatment, while ovalbumin administration did not affect swelling of the nasal mucosa in either group. This UAHR following MCh treatment was completely reversed by pretreatment with glucocorticoids. This novel approach using micro-CT for investigating UAHR reflects a precise assessment system for swelling of the nasal mucosa following MCh treatment; it not only sheds light on the mechanism of AR but also contributes to the development of new therapeutic drugs in AR patients. View Full-Text
Keywords: Allergic rhinitis; airway hyperresponsiveness (AHR); eosinophilic airway inflammation; micro-CT; and nasal resistance Allergic rhinitis; airway hyperresponsiveness (AHR); eosinophilic airway inflammation; micro-CT; and nasal resistance
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Bui, D.V.; Kanda, A.; Kobayashi, Y.; Sakata, Y.; Kono, Y.; Kamakura, Y.; Jinno, T.; Yun, Y.; Suzuki, K.; Sawada, S.; Asako, M.; Nakamura, A.; Dombrowicz, D.; Utsunomiya, K.; Noboru, T.; Tomoda, K.; Iwai, H. A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model. Biomolecules 2019, 9, 252.

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