Next Article in Journal
A Novel Approach for Investigating Upper Airway Hyperresponsiveness Using Micro-CT in Eosinophilic Upper Airway Inflammation such as Allergic Rhinitis Model
Previous Article in Journal
Misfolding of a Single Disulfide Bonded Globular Protein into a Low-Solubility Species Conformationally and Biophysically Distinct from the Native One
Open AccessArticle

Correlating Lipid Membrane Permeabilities of Imidazolium Ionic Liquids with Their Cytotoxicities on Yeast, Bacterial, and Mammalian Cells

1
Department of Chemistry and Biochemistry, Rowan University, Glassboro, NJ 08028, USA
2
Department of Molecular and Cellular Biosciences, Rowan University, Glassboro, NJ 08028, USA
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(6), 251; https://doi.org/10.3390/biom9060251
Received: 6 June 2019 / Revised: 19 June 2019 / Accepted: 21 June 2019 / Published: 25 June 2019
(This article belongs to the Section Biochemistry)
Alkyl-imidazolium chloride ionic liquids (ILs) have been broadly studied for biochemical and biomedical technologies. They can permeabilize lipid bilayer membranes and have cytotoxic effects, which makes them targets for drug delivery biomaterials. We assessed the lipid-membrane permeabilities of ILs with increasing alkyl chain lengths from ethyl to octyl groups on large unilamellar vesicles using a trapped-fluorophore fluorescence lifetime-based leakage experiment. Only the most hydrophobic IL, with the octyl chain, permeabilizes vesicles, and the concentration required for permeabilization corresponds to its critical micelle concentration. To correlate the model vesicle studies with biological cells, we quantified the IL permeabilities and cytotoxicities on different cell lines including bacterial, yeast, and ovine blood cells. The IL permeabilities on vesicles strongly correlate with permeabilities and minimum inhibitory concentrations on biological cells. Despite exhibiting a broad range of lipid compositions, the ILs appear to have similar effects on the vesicles and cell membranes. View Full-Text
Keywords: ionic liquids; lipids; permeability; cytotoxicity; leakage assay; flow cytometry ionic liquids; lipids; permeability; cytotoxicity; leakage assay; flow cytometry
Show Figures

Graphical abstract

MDPI and ACS Style

Cook, K.; Tarnawsky, K.; Swinton, A.J.; Yang, D.D.; Senetra, A.S.; Caputo, G.A.; Carone, B.R.; Vaden, T.D. Correlating Lipid Membrane Permeabilities of Imidazolium Ionic Liquids with Their Cytotoxicities on Yeast, Bacterial, and Mammalian Cells. Biomolecules 2019, 9, 251.

AMA Style

Cook K, Tarnawsky K, Swinton AJ, Yang DD, Senetra AS, Caputo GA, Carone BR, Vaden TD. Correlating Lipid Membrane Permeabilities of Imidazolium Ionic Liquids with Their Cytotoxicities on Yeast, Bacterial, and Mammalian Cells. Biomolecules. 2019; 9(6):251.

Chicago/Turabian Style

Cook, Kendall; Tarnawsky, Katharine; Swinton, Alana J.; Yang, Daniel D.; Senetra, Alexandria S.; Caputo, Gregory A.; Carone, Benjamin R.; Vaden, Timothy D. 2019. "Correlating Lipid Membrane Permeabilities of Imidazolium Ionic Liquids with Their Cytotoxicities on Yeast, Bacterial, and Mammalian Cells" Biomolecules 9, no. 6: 251.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop