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Open AccessArticle

Extraction of Cathepsin D-Like Protease from Neon Flying Squid (Ommastrephes bartramii) Viscera and Application in Antioxidant Hydrolysate Production

1
School of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China
2
School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(6), 228; https://doi.org/10.3390/biom9060228
Received: 24 May 2019 / Revised: 9 June 2019 / Accepted: 9 June 2019 / Published: 12 June 2019
(This article belongs to the Special Issue Bioactives from Marine Products)
A protease from neon flying squid (Ommastrephes bartramii) viscera (SVCE3(f)) was partially purified by isoelectric solubilization/precipitation combined with ultra-membrane filtration (ISP-UMF). Two protein bands of 45 and 27 KDa were determined by SDS-PAGE assay. The protease characteristic of the protein band of 45 KDa was confirmed using casein zymography analysis. The result of UPLC-ESI-MS/MS suggested that the band of 45 KDa could be a cathepsin D-like protease. This cathepsin D-like protease showed an optimum pH of 3.0 and optimum temperature of 60 °C when casein was used as s substrate. Furthermore, its protease activity was stable at 30–50 °C and under a pH range of 1.0–5.0, maintaining about 60% of its initial activity. SVCE3(f) can digest half-fin anchovy (Setipinna taty) to generate antioxidant hydrolysates (HAHp-SEs). The degree of hydrolysis (DH) of HAHp-SEs increased along with the hydrolysis time and reached stability after 60 min of digestion. HAHp-SEs(30) with relatively lower DH exhibited the highest DPPH radical scavenging activity as compared with other HAHp-SEs. However, a stronger hydroxyl radical scavenging activity and greater reducing power were observed for HAHp-SEs that underwent higher DH. Accordingly, the partially purified cathepsin D-like protease of neon flying squid viscera using ISP-UMF could have potential application in antioxidant hydrolysates production. View Full-Text
Keywords: neon flying squid viscera; isoelectric solubilization/precipitation; ultra-membrane filtration; cathepsin D-like protease; property; half-fin anchovy (Setipinna taty) hydrolysates; antioxidant neon flying squid viscera; isoelectric solubilization/precipitation; ultra-membrane filtration; cathepsin D-like protease; property; half-fin anchovy (Setipinna taty) hydrolysates; antioxidant
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MDPI and ACS Style

Zhang, K.; Wei, R.; Song, R. Extraction of Cathepsin D-Like Protease from Neon Flying Squid (Ommastrephes bartramii) Viscera and Application in Antioxidant Hydrolysate Production. Biomolecules 2019, 9, 228. https://doi.org/10.3390/biom9060228

AMA Style

Zhang K, Wei R, Song R. Extraction of Cathepsin D-Like Protease from Neon Flying Squid (Ommastrephes bartramii) Viscera and Application in Antioxidant Hydrolysate Production. Biomolecules. 2019; 9(6):228. https://doi.org/10.3390/biom9060228

Chicago/Turabian Style

Zhang, Kaiqiang; Wei, Rongbian; Song, Ru. 2019. "Extraction of Cathepsin D-Like Protease from Neon Flying Squid (Ommastrephes bartramii) Viscera and Application in Antioxidant Hydrolysate Production" Biomolecules 9, no. 6: 228. https://doi.org/10.3390/biom9060228

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