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Biomolecules 2019, 9(2), 66; https://doi.org/10.3390/biom9020066

Genome-Wide Identification and Characterization of SQUAMOSA—Promoter-Binding Protein (SBP) Genes Involved in the Flowering Development of Citrus Clementina

1
Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China
2
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230000, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 25 December 2018 / Revised: 7 February 2019 / Accepted: 7 February 2019 / Published: 14 February 2019
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Abstract

SQUAMOSA-promoter binding protein (SBP)-box genes encode a family of plant-specific transcription factors that play vital roles in plant growth and development. In this study, 15 SBP-box genes were identified and isolated from Citrus clementina (CclSBPs), where 10 of these genes were predicted to be putative targets of Citrus clementina microRNA156 (CclmiR156). The 15 CclSBP genes could be classified into six groups based on phylogenetic analysis, diverse intron–exon structure, and motif prediction, similar to the SQUAMOSA promoter binding protein-like (SPL) gene family of Populus trichocarpa and Arabidopsis thaliana. Furthermore, CclSBPs classified into a group/subgroup have similar gene structures and conserved motifs, implying their functional redundancy. Tissue-specific expression analysis of CclSBPs demonstrated their diversified expression patterns. To further explore the potential role of CclSBPs during floral inductive water deficits, the dynamic changes of the 15 CclSBPs were investigated during floral inductive water deficits, and the results showed that some CclSBPs were associated with floral induction. Among these genes, CclSBP6 was not homologous to the Arabidopsis SBP-box gene family, and CclSBP7 was regulated by being alternatively spliced. Therefore, CclSBP6 and CclSBP7 were genetically transformed in Arabidopsis. Overexpression of the two genes changed the flowering time of Arabidopsis. View Full-Text
Keywords: Citrus clementina; SBP-box genes; gene expression; evolutionary comparison; functional diversity Citrus clementina; SBP-box genes; gene expression; evolutionary comparison; functional diversity
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    Link: http://Figure S1, Table S1 and Table S2
    Description: Supplementary Materials: Figure S1. Functional diversity analysis of SBP-box genes in other plants, and phylogenetic relationship with citrus SBP-box genes. Phylogeny of SBP-box genes based on an alignment of the SBP-box domain, only genes for which functional data were available selected from other plant species. Green circles, presence; white circles, absence; black circles, unknown. Table S1 Gene-specific primers used in this study. Table S2. Cis-regulatory elements predicted in promoter region of CclSBPs.
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Zeng, R.-F.; Zhou, J.-J.; Liu, S.-R.; Gan, Z.-M.; Zhang, J.-Z.; Hu, C.-G. Genome-Wide Identification and Characterization of SQUAMOSA—Promoter-Binding Protein (SBP) Genes Involved in the Flowering Development of Citrus Clementina. Biomolecules 2019, 9, 66.

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