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Open AccessArticle

Effects of Synergistic Inhibition on α-glucosidase by Phytoalexins in Soybeans

1
School of Food Science & Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea
2
Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USA
3
Korea Research Institute of Chemical Technology, Daejeon 34114, Korea
4
Biomedical Research Institute, Kyungpook National University Hospital, Daegu 41940, Korea
5
Major in Biomodulation, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
6
School of Life Sciences, KNU Creative BioResearch Group, Institute of Microorganisms, Kyungpook National University, Daegu 41566, Korea
7
knu BnC, Daegu 41566, Korea
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 828; https://doi.org/10.3390/biom9120828
Received: 18 October 2019 / Revised: 2 December 2019 / Accepted: 3 December 2019 / Published: 5 December 2019
(This article belongs to the Special Issue 2019 Feature Papers by Biomolecules’ Editorial Board Members)
To determine the mechanism of action of the effects of phytoalexins in soybeans, we analyzed α-glucosidase inhibition kinetics using Michaelis–Menten plots and Lineweaver–Burk plots. The results showed that the type of inhibition with glyceollin was competitive, that of genistein was noncompetitive, that of daidzein was uncompetitive, and luteolin showed a mixed mode of action. The Ki values were determined using a Dixon plot as glyceollin, 18.99 μM; genistein, 15.42 μM; luteolin, 16.81 μM; and daidzein, 9.99 μM. Furthermore, potential synergistic effects between glyceollin and the three polyphenols were investigated. A combination of glyceollin and luteolin at a ratio of 3:7 exhibited synergistic effects on α-glucosidase inhibition, having a combination index (CI) of 0.64244, according to the CI–isobologram equation. Collectively, these results showed that a combination of glyceollin and luteolin has the potential to inhibit α-glucosidase activity via a synergistic mode of inhibition. View Full-Text
Keywords: α-glucosidase inhibitor; glyceollin; genistein; luteolin; daidzein; phytoalexins; enzyme kinetics; combination index α-glucosidase inhibitor; glyceollin; genistein; luteolin; daidzein; phytoalexins; enzyme kinetics; combination index
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MDPI and ACS Style

Son, H.-U.; Yoon, E.-K.; Yoo, C.-Y.; Park, C.-H.; Bae, M.-A.; Kim, T.-H.; Lee, C.H.; Lee, K.W.; Seo, H.; Kim, K.-J.; Lee, S.-H. Effects of Synergistic Inhibition on α-glucosidase by Phytoalexins in Soybeans. Biomolecules 2019, 9, 828.

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