Next Article in Journal
Interaction of a Polyarginine Peptide with Membranes of Different Mechanical Properties
Next Article in Special Issue
Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
Previous Article in Journal
Epigenetic Regulation of Auxin Homeostasis
Previous Article in Special Issue
Effect of Marination Time on the Antioxidant Properties of Peptides Extracted from Organic Dry-Fermented Beef
Open AccessArticle

Electro-Acupuncture Alleviates Cisplatin-Induced Anorexia in Rats by Modulating Ghrelin and Monoamine Neurotransmitters

1
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
2
Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Korea
3
College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam 13488, Korea
4
Korean Medicine Clinical Trial Center, Korean Medicine Hospital, Kyung Hee University, Seoul 02447, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the work described in this study.
Biomolecules 2019, 9(10), 624; https://doi.org/10.3390/biom9100624
Received: 12 September 2019 / Revised: 8 October 2019 / Accepted: 15 October 2019 / Published: 18 October 2019
(This article belongs to the Special Issue Peptides: Molecular and Biotechnological Aspects)
Anorexia is common in patients with cancer, mostly as a side effect of chemotherapy. The effect of electro-acupuncture (EA) on ameliorating cancer-related symptoms have been studied in animal models and in clinical trials. The aim of this study was to determine optimal conditions for the application of EA to alleviate anorexia, followed by the study of molecular mechanisms affecting its therapeutics. Anorexia was induced in male Wistar rats by injecting cisplatin, which was then followed by EA treatment at CV12, the acupuncture point located in the center of the abdominal midline. Body weight and food intake were measured daily throughout the duration of the study. The levels of monoamine neurotransmitters in the plasma were quantitatively analyzed by HPLC-ECD. Gastrointestinal hormone concentrations were elucidated with ELISA kits. RT-qPCR was performed to evaluate the mRNA expression of ghrelin (GHRL), neuropeptide Y (NPY), and pro-opiomelanocortin. The expression of c-Fos in the nucleus tractus solitarii was detected using western blotting analysis. The optimal conditions of EA to alleviate anorexia in rats was determined to be 1 unit for intensity and 10 Hz for frequency. EA treatment at CV12 reduced the levels of plasma monoamine neurotransmitters 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, dopamine, and norepinephrine; as well as stimulated the expression of GHRL and NPY to alleviate cisplatin-induced anorexia in rats. EA stimulation at CV12 could be used to treat cisplatin-induced anorexia in rats. View Full-Text
Keywords: electro-acupuncture; 5-hydroxytryptamine; ghrelin; neuropeptide Y; pro-opiomelanocortin; cisplatin; anorexia; cancer electro-acupuncture; 5-hydroxytryptamine; ghrelin; neuropeptide Y; pro-opiomelanocortin; cisplatin; anorexia; cancer
Show Figures

Graphical abstract

MDPI and ACS Style

Baek, J.Y.; Trinh, T.A.; Huh, W.; Song, J.H.; Kim, H.Y.; Lim, J.; Kim, J.; Choi, H.J.; Kim, T.-H.; Kang, K.S. Electro-Acupuncture Alleviates Cisplatin-Induced Anorexia in Rats by Modulating Ghrelin and Monoamine Neurotransmitters. Biomolecules 2019, 9, 624.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop