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Article

Metabolite Profiling in Arabidopsisthaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration

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Plant Physiology Department, University of Rostock, Albert-Einstein-Straße 3, 18059 Rostock, Germany
2
Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Golm, Germany
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Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa 36570-900, Minas Gerais, Brazil
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Institute of Plant Biochemistry, Cluster of Excellence on Plant Science (CEPLAS), Heinrich Heine University Düsseldorf, Universitätsstrasse 1, 40225 Düsseldorf, Germany
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Centre for Organismal Studies, University of Heidelberg, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany
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Department of Molecular Systems Biology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria
*
Authors to whom correspondence should be addressed.
Academic Editor: Mathieu Jossier
Metabolites 2021, 11(6), 391; https://doi.org/10.3390/metabo11060391
Received: 25 May 2021 / Revised: 9 June 2021 / Accepted: 11 June 2021 / Published: 15 June 2021
(This article belongs to the Special Issue Photorespiration on Plant Metabolism)
Photorespiration is an integral component of plant primary metabolism. Accordingly, it has been often observed that impairing the photorespiratory flux negatively impacts other cellular processes. In this study, the metabolic acclimation of the Arabidopsisthaliana wild type was compared with the hydroxypyruvate reductase 1 (HPR1; hpr1) mutant, displaying only a moderately reduced photorespiratory flux. Plants were analyzed during development and under varying photoperiods with a combination of non-targeted and targeted metabolome analysis, as well as 13C- and 14C-labeling approaches. The results showed that HPR1 deficiency is more critical for photorespiration during the vegetative compared to the regenerative growth phase. A shorter photoperiod seems to slowdown the photorespiratory metabolite conversion mostly at the glycerate kinase and glycine decarboxylase steps compared to long days. It is demonstrated that even a moderate impairment of photorespiration severely reduces the leaf-carbohydrate status and impacts on sulfur metabolism. Isotope labeling approaches revealed an increased CO2 release from hpr1 leaves, most likely occurring from enhanced non-enzymatic 3-hydroxypyruvate decarboxylation and a higher flux from serine towards ethanolamine through serine decarboxylase. Collectively, the study provides evidence that the moderate hpr1 mutant is an excellent tool to unravel the underlying mechanisms governing the regulation of metabolic linkages of photorespiration with plant primary metabolism. View Full-Text
Keywords: Arabidopsis; photorespiration; hydroxypyruvate reductase; metabolomics; isotope labeling; metabolic acclimation; plant development; photoperiodic acclimation Arabidopsis; photorespiration; hydroxypyruvate reductase; metabolomics; isotope labeling; metabolic acclimation; plant development; photoperiodic acclimation
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MDPI and ACS Style

Timm, S.; Nunes-Nesi, A.; Florian, A.; Eisenhut, M.; Morgenthal, K.; Wirtz, M.; Hell, R.; Weckwerth, W.; Hagemann, M.; Fernie, A.R.; Bauwe, H. Metabolite Profiling in Arabidopsisthaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration. Metabolites 2021, 11, 391. https://doi.org/10.3390/metabo11060391

AMA Style

Timm S, Nunes-Nesi A, Florian A, Eisenhut M, Morgenthal K, Wirtz M, Hell R, Weckwerth W, Hagemann M, Fernie AR, Bauwe H. Metabolite Profiling in Arabidopsisthaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration. Metabolites. 2021; 11(6):391. https://doi.org/10.3390/metabo11060391

Chicago/Turabian Style

Timm, Stefan, Adriano Nunes-Nesi, Alexandra Florian, Marion Eisenhut, Katja Morgenthal, Markus Wirtz, Rüdiger Hell, Wolfram Weckwerth, Martin Hagemann, Alisdair R. Fernie, and Hermann Bauwe. 2021. "Metabolite Profiling in Arabidopsisthaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration" Metabolites 11, no. 6: 391. https://doi.org/10.3390/metabo11060391

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