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Article

Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry

1
Department of Chemistry, Stanford University, Stanford, CA 94305, USA
2
Department of Chemistry, Fudan University, Shanghai 200438, China
*
Author to whom correspondence should be addressed.
Academic Editors: Peter Oefner and Zoltan Takats
Metabolites 2021, 11(11), 728; https://doi.org/10.3390/metabo11110728
Received: 1 October 2021 / Revised: 21 October 2021 / Accepted: 22 October 2021 / Published: 23 October 2021
(This article belongs to the Special Issue Advances in Ambient Ionization Techniques for Mass Spectrometry)
Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer. View Full-Text
Keywords: hydrogen-deuterium exchange; isobaric ions; ambient ionization mass spectrometry; microdroplets hydrogen-deuterium exchange; isobaric ions; ambient ionization mass spectrometry; microdroplets
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MDPI and ACS Style

Song, X.; Li, J.; Mofidfar, M.; Zare, R.N. Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry. Metabolites 2021, 11, 728. https://doi.org/10.3390/metabo11110728

AMA Style

Song X, Li J, Mofidfar M, Zare RN. Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry. Metabolites. 2021; 11(11):728. https://doi.org/10.3390/metabo11110728

Chicago/Turabian Style

Song, Xiaowei, Jia Li, Mohammad Mofidfar, and Richard N. Zare 2021. "Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry" Metabolites 11, no. 11: 728. https://doi.org/10.3390/metabo11110728

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