Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Rapid Recognition of Opioid Narcotics
2.2. Distinguishing Isobaric Ions That Are Challenging to Tell Apart by MS/MS
2.3. Correcting Falsely Matched Adduct Ions
2.4. Comparison with Gas-Phase HDX
2.5. Creating New Dimension and Features for Metabolic Profiling
2.6. Relative and Absolute Quantitation
3. Methods
3.1. Reagents and Materials
3.2. Solution Preparation and Bio-Sample Collection
3.3. Droplet Spray Ionization
3.4. Data Acquisition and Processing
3.5. Isobaric Ion Discrimination and Assignment
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Song, X.; Li, J.; Mofidfar, M.; Zare, R.N. Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry. Metabolites 2021, 11, 728. https://doi.org/10.3390/metabo11110728
Song X, Li J, Mofidfar M, Zare RN. Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry. Metabolites. 2021; 11(11):728. https://doi.org/10.3390/metabo11110728
Chicago/Turabian StyleSong, Xiaowei, Jia Li, Mohammad Mofidfar, and Richard N. Zare. 2021. "Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry" Metabolites 11, no. 11: 728. https://doi.org/10.3390/metabo11110728