Next Article in Journal
Towards Standardization of Data Normalization Strategies to Improve Urinary Metabolomics Studies by GC×GC-TOFMS
Previous Article in Journal
Metabolomic Profiling in Neuromyelitis Optica Spectrum Disorder Biomarker Discovery
Previous Article in Special Issue
Lipidomics Issues on Human Positive ssRNA Virus Infection: An Update
Article

Chasing the Major Sphingolipids on Earth: Automated Annotation of Plant Glycosyl Inositol Phospho Ceramides by Glycolipidomics

1
Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Str. 38, 1090 Vienna, Austria
2
Vienna Metabolomics Center (VIME), University of Vienna, Althanstraße 14, 1090 Vienna, Austria
3
Chemistry Meets Microbiology, University of Vienna, Althanstraße 14, 1090 Vienna, Austria
4
Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 1/I, 8010 Graz, Austria
*
Author to whom correspondence should be addressed.
Metabolites 2020, 10(9), 375; https://doi.org/10.3390/metabo10090375
Received: 20 August 2020 / Revised: 16 September 2020 / Accepted: 16 September 2020 / Published: 19 September 2020
(This article belongs to the Special Issue Advances in Lipidomics: Biomedicine, Nutrients and Methodology)
Glycosyl inositol phospho ceramides (GIPCs) are the major sphingolipids on earth, as they account for a considerable fraction of the total lipids in plants and fungi, which in turn represent a large portion of the biomass on earth. Despite their obvious importance, GIPC analysis remains challenging due to the lack of commercial standards and automated annotation software. In this work, we introduce a novel GIPC glycolipidomics workflow based on reversed-phase ultra-high pressure liquid chromatography coupled to high-resolution mass spectrometry. For the first time, automated GIPC assignment was performed using the open-source software Lipid Data Analyzer (LDA), based on platform-independent decision rules. Four different plant samples (salad, spinach, raspberry, and strawberry) were analyzed and the results revealed 64 GIPCs based on accurate mass, characteristic MS2 fragments and matching retention times. Relative quantification using lactosyl ceramide for internal standardization revealed GIPC t18:1/h24:0 as the most abundant species in all plants. Depending on the plant sample, GIPCs contained mainly amine, N-acetylamine or hydroxyl residues. Most GIPCs revealed a Hex-HexA-IPC core and contained a ceramide part with a trihydroxylated t18:0 or a t18:1 long chain base and hydroxylated fatty acid chains ranging from 16 to 26 carbon atoms in length (h16:0–h26:0). Interestingly, four GIPCs containing t18:2 were observed in the raspberry sample, which was not reported so far. The presented workflow supports the characterization of different plant samples by automatic GIPC assignment, potentially leading to the identification of new GIPCs. For the first time, automated high-throughput profiling of these complex glycolipids is possible by liquid chromatography-high-resolution tandem mass spectrometry and subsequent automated glycolipid annotation based on decision rules. View Full-Text
Keywords: glycolipidomics; GIPC; glycosyl inositol phospho ceramides; Lipid Data Analyzer; lipidomics; sphingolipids; ultra-high pressure liquid chromatography; high-resolution mass spectrometry; LC-MS; automated annotation glycolipidomics; GIPC; glycosyl inositol phospho ceramides; Lipid Data Analyzer; lipidomics; sphingolipids; ultra-high pressure liquid chromatography; high-resolution mass spectrometry; LC-MS; automated annotation
Show Figures

Graphical abstract

MDPI and ACS Style

Panzenboeck, L.; Troppmair, N.; Schlachter, S.; Koellensperger, G.; Hartler, J.; Rampler, E. Chasing the Major Sphingolipids on Earth: Automated Annotation of Plant Glycosyl Inositol Phospho Ceramides by Glycolipidomics. Metabolites 2020, 10, 375. https://doi.org/10.3390/metabo10090375

AMA Style

Panzenboeck L, Troppmair N, Schlachter S, Koellensperger G, Hartler J, Rampler E. Chasing the Major Sphingolipids on Earth: Automated Annotation of Plant Glycosyl Inositol Phospho Ceramides by Glycolipidomics. Metabolites. 2020; 10(9):375. https://doi.org/10.3390/metabo10090375

Chicago/Turabian Style

Panzenboeck, Lisa, Nina Troppmair, Sara Schlachter, Gunda Koellensperger, Jürgen Hartler, and Evelyn Rampler. 2020. "Chasing the Major Sphingolipids on Earth: Automated Annotation of Plant Glycosyl Inositol Phospho Ceramides by Glycolipidomics" Metabolites 10, no. 9: 375. https://doi.org/10.3390/metabo10090375

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop