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Open AccessReview

Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae

by 1,†, 1,†, 1,†, 1,†, 1, 2, 1,* and 1,3,4,5,6,*
1
Center for Synthetic Biochemistry, Shenzhen Institutes for Advanced Technologies, Chinese Academy of Sciences, Shenzhen 518055, China
2
School of Pharmaceutical Sciences, Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou 450001, China
3
Joint BioEnergy Institute, Emeryville, CA 94608, USA
4
Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
5
Department of Chemical and Biomolecular Engineering & Department of Bioengineering, University of California, Berkeley, CA 94720, USA
6
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Metabolites 2020, 10(8), 320; https://doi.org/10.3390/metabo10080320
Received: 30 June 2020 / Revised: 30 July 2020 / Accepted: 4 August 2020 / Published: 6 August 2020
(This article belongs to the Special Issue Metabolic Engineering and Synthetic Biology Volume 2)
Promoters play an essential role in the regulation of gene expression for fine-tuning genetic circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However, native promoters in S. cerevisiae have several limitations which hinder their applications in metabolic engineering. These limitations include an inadequate number of well-characterized promoters, poor dynamic range, and insufficient orthogonality to endogenous regulations. Therefore, it is necessary to perform promoter engineering to create synthetic promoters with better properties. Here, we review recent advances related to promoter architecture, promoter engineering and synthetic promoter applications in S. cerevisiae. We also provide a perspective of future directions in this field with an emphasis on the recent advances of machine learning based promoter designs. View Full-Text
Keywords: promoter architecture; promoter engineering; synthetic promoter; synthetic biology; machine learning; Saccharomyces cerevisiae promoter architecture; promoter engineering; synthetic promoter; synthetic biology; machine learning; Saccharomyces cerevisiae
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MDPI and ACS Style

Tang, H.; Wu, Y.; Deng, J.; Chen, N.; Zheng, Z.; Wei, Y.; Luo, X.; Keasling, J.D. Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae. Metabolites 2020, 10, 320. https://doi.org/10.3390/metabo10080320

AMA Style

Tang H, Wu Y, Deng J, Chen N, Zheng Z, Wei Y, Luo X, Keasling JD. Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae. Metabolites. 2020; 10(8):320. https://doi.org/10.3390/metabo10080320

Chicago/Turabian Style

Tang, Hongting; Wu, Yanling; Deng, Jiliang; Chen, Nanzhu; Zheng, Zhaohui; Wei, Yongjun; Luo, Xiaozhou; Keasling, Jay D. 2020. "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae" Metabolites 10, no. 8: 320. https://doi.org/10.3390/metabo10080320

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