Next Article in Journal
Effects of Thymoquinone on Small-Molecule Metabolites in a Rat Model of Cerebral Ischemia Reperfusion Injury Assessed using MALDI-MSI
Previous Article in Journal
Erratum: Ismail, I.T.; et al. Inborn Errors of Metabolism in the Era of Untargeted Metabolomics and Lipidomics. Metabolites 2019, 9, 242
Previous Article in Special Issue
Proton NMR Enables the Absolute Quantification of Aqueous Metabolites and Lipid Classes in Unique Mouse Liver Samples
Open AccessArticle

Targeted Analysis of 46 Bile Acids to Study the Effect of Acetaminophen in Rat by LC-MS/MS

Department of Chemistry, Université du Québec à Montréal (UQAM), P.O. Box 8888 Downtown Station, Montreal, QC H3C 3P8, Canada
*
Author to whom correspondence should be addressed.
Metabolites 2020, 10(1), 26; https://doi.org/10.3390/metabo10010026
Received: 30 September 2019 / Revised: 30 December 2019 / Accepted: 6 January 2020 / Published: 7 January 2020
(This article belongs to the Special Issue Metabolism and Metabolomics of Liver in Health and Disease)
Bile acids represent a large class of steroid acids synthesized in the liver and further metabolized by many bacterial and mammalian enzymes. Variations in bile acid levels can be used as a measure of liver function. There still exists, however, a need to study the variation of individual circulating bile acids in the context of hepatotoxity or liver disease. Acetaminophen (APAP), a drug commonly taken to relieve pain and decrease fever, is known to cause acute liver failure at high doses. We have developed a targeted liquid chromatography-tandem mass spectrometry method to monitor the effects of different doses of APAP on the bile acid plasma profile in a rat model. The analysis method was optimized to ensure chromatographic resolution of isomeric species using a mixture of 46 standard bile acids, and 14 isotopically-labeled internal standard (IS) compounds detected in multiple reaction monitoring (MRM) mode on a triple quadrupole mass spectrometer. Four doses of acetaminophen were studied, the highest of which shows signs of hepatotoxicity in rats. This targeted method revealed that high dose APAP has an important effect on bile acid profiles. Changes were seen in several unconjugated bile acids as well as glycine conjugates; however, no obvious changes were apparent for taurine-conjugated species. View Full-Text
Keywords: bile acids; metabolomics; rat plasma; tandem mass spectrometry; liquid chromatography; acetaminophen; hepatotoxicity bile acids; metabolomics; rat plasma; tandem mass spectrometry; liquid chromatography; acetaminophen; hepatotoxicity
Show Figures

Figure 1

MDPI and ACS Style

Prinville, V.; Ohlund, L.; Sleno, L. Targeted Analysis of 46 Bile Acids to Study the Effect of Acetaminophen in Rat by LC-MS/MS. Metabolites 2020, 10, 26.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop