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Metabolomics Provide Sensitive Insights into the Impacts of Low Level Environmental Contamination on Fish Health—A Pilot Study

1
Centre for Aquatic Pollution Identification and Management (CAPIM), Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Road, Parkville, VIC 3010, Australia
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Aquatic Environmental Stress (AQUEST) Research Group, School of Science, RMIT University, PO Box 71, Bundoora, VIC 3083, Australia
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Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Road, Melbourne, VIC 3010, Australia
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Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, 30 Flemington Road, Parkville, VIC 3010, Australia
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Centre for Aquatic Pollution Identification and Management (CAPIM), The University of Melbourne, Parkville, VIC 3010, Australia
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School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia
*
Author to whom correspondence should be addressed.
Present address: Trajan Scientific and Medical, 7 Argent Pl, Ringwood, VIC 3134, Australia.
Present address: CSL limited, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC 3010, Australia.
Metabolites 2020, 10(1), 24; https://doi.org/10.3390/metabo10010024
Received: 28 November 2019 / Revised: 23 December 2019 / Accepted: 2 January 2020 / Published: 6 January 2020
This exploratory study aims to investigate the health of sand flathead (Platycephalus bassensis) sampled from five sites in Port Phillip Bay, Australia using gas chromatography-mass spectrometry (GC-MS) metabolomics approaches. Three of the sites were the recipients of industrial, agricultural, and urban run-off and were considered urban sites, while the remaining two sites were remote from contaminant inputs, and hence classed as rural sites. Morphological parameters as well as polar and free fatty acid metabolites were used to investigate inter-site differences in fish health. Significant differences in liver somatic index (LSI) and metabolite abundance were observed between the urban and rural sites. Differences included higher LSI, an increased abundance of amino acids and energy metabolites, and reduced abundance of free fatty acids at the urban sites compared to the rural sites. These differences might be related to the additional energy requirements needed to cope with low-level contaminant exposure through energy demanding processes such as detoxification and antioxidant responses as well as differences in diet between the sites. In this study, we demonstrate that metabolomics approaches can offer a greater level of sensitivity compared to traditional parameters such as physiological parameters or biochemical markers of fish health, most of which showed no or little inter-site differences in the present study. Moreover, the metabolite responses are more informative than traditional biomarkers in terms of biological significance as disturbances in specific metabolic pathways can be identified. View Full-Text
Keywords: biomarkers; fatty acid metabolites; energy metabolism; amino acids; metals; diet; flathead; Platycephalus bassensis biomarkers; fatty acid metabolites; energy metabolism; amino acids; metals; diet; flathead; Platycephalus bassensis
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Long, S.M.; Tull, D.L.; De Souza, D.P.; Kouremenos, K.A.; Dayalan, S.; McConville, M.J.; Hassell, K.L.; Pettigrove, V.J.; Gagnon, M.M. Metabolomics Provide Sensitive Insights into the Impacts of Low Level Environmental Contamination on Fish Health—A Pilot Study. Metabolites 2020, 10, 24.

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