Direct Implementation of Intestinal Permeability Test in NMR Metabolomics for Simultaneous Biomarker Discovery—A Feasibility Study in a Preterm Piglet Model

Alinaghi, Masoumeh; Nguyen, Duc Ninh; Sangild, Per Torp; Bertram, Hanne Christine

doi:10.3390/metabo10010022

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

Direct Implementation of Intestinal Permeability Test in NMR Metabolomics for Simultaneous Biomarker Discovery—A Feasibility Study in a Preterm Piglet Model

Masoumeh Alinaghi

¹,

Duc Ninh Nguyen

Per Torp Sangild

² and

Hanne Christine Bertram

^1,*

Department of Food Science, Aarhus University, Kirstinebjergvej 10, 5792 Aarslev, Denmark

Section for Comparative Pediatrics and Nutrition, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlaegevej 68, 1860 Frederiksberg C, Denmark

Author to whom correspondence should be addressed.

Metabolites 2020, 10(1), 22; https://doi.org/10.3390/metabo10010022

Received: 24 October 2019 / Revised: 22 December 2019 / Accepted: 29 December 2019 / Published: 1 January 2020

(This article belongs to the Special Issue Metabolomics Methodologies and Applications)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Measurement of intestinal permeability (IP) is often used in the examination of inflammatory gastrointestinal disorders. IP can be assessed by measurement of urinary recovery of ingested non-metabolizable lactulose (L) and mannitol (M). The present study aimed to examine how measurements of IP can be integrated in a NMR-based metabolomics approach for a simultaneous quantification of L/M ratio and biomarker exploration. For this purpose, plasma and urine samples were collected from five-day-old preterm piglets (n = 20) with gastrointestinal disorders (subjected to intra-amniotic lipopolysaccharide (LPS, 1 mg/fetus)) after they had been administrated a 5% lactulose and 5% mannitol solution (15 mL/kg). The collected plasma and urine samples were analyzed by ¹H NMR-based metabolomics. Urine L/M ratio measured by ¹H NMR spectroscopy showed high correlation with the standard measurement of the urinary recoveries by enzymatic assays (r = 0.93, p < 0.05). Partial least squares (PLS) regressions and correlation analyses between L/M ratio and NMR metabolomics data revealed that L/M ratio was positively correlated with plasma lactate, acetate and succinate levels and negatively correlated with urinary hippuric acid and glycine. In conclusion, the present study demonstrated that NMR metabolomics enables simultaneous IP testing and discovery of biomarkers associated with an impaired intestinal permeability. View Full-Text

Keywords: intestinal barrier dysfunction; leaky gut; preterm infants; lipopolysaccharide (LPS); prenatal inflammation; NMR metabolomics; lactulose

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

Supplementary File 1:
PDF-Document (PDF, 89 KiB)

Share and Cite

MDPI and ACS Style

Alinaghi, M.; Nguyen, D.N.; Sangild, P.T.; Bertram, H.C. Direct Implementation of Intestinal Permeability Test in NMR Metabolomics for Simultaneous Biomarker Discovery—A Feasibility Study in a Preterm Piglet Model. Metabolites 2020, 10, 22. https://doi.org/10.3390/metabo10010022

AMA Style

Alinaghi M, Nguyen DN, Sangild PT, Bertram HC. Direct Implementation of Intestinal Permeability Test in NMR Metabolomics for Simultaneous Biomarker Discovery—A Feasibility Study in a Preterm Piglet Model. Metabolites. 2020; 10(1):22. https://doi.org/10.3390/metabo10010022

Chicago/Turabian Style

Alinaghi, Masoumeh, Duc N. Nguyen, Per T. Sangild, and Hanne C. Bertram. 2020. "Direct Implementation of Intestinal Permeability Test in NMR Metabolomics for Simultaneous Biomarker Discovery—A Feasibility Study in a Preterm Piglet Model" Metabolites 10, no. 1: 22. https://doi.org/10.3390/metabo10010022

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

Direct Implementation of Intestinal Permeability Test in NMR Metabolomics for Simultaneous Biomarker Discovery—A Feasibility Study in a Preterm Piglet Model

Abstract

Supplementary Material

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI