Next Article in Journal
Development of an Ion Chromatography Method for Analysis of Organic Anions (Fumarate, Oxalate, Succinate, and Tartrate) in Single Chromatographic Conditions
Previous Article in Journal
Development and Validation of a Stability-Indicating RP-UPLC Method for the Estimation of Impurities in Cinacalcet Hydrochloride API and its Formulation
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sci. Pharm.
Volume 83
Issue 4
10.3797/scipharm.1505-03
scipharm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA

by
Chetan SHARMA
,
Pragya Nand BADYAL
and
Ravindra K. RAWAL
*
Department of Pharmaceutical Analysis, Indo-Soviet Friendship College of Pharmacy (ISFCP), Moga 142001, India
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2015, 83(4), 599-610; https://doi.org/10.3797/scipharm.1505-03
Submission received: 5 May 2015 / Accepted: 22 June 2015 / Published: 22 June 2015
Versions Notes

Abstract

In this study, new and feasible UV-visible spectrophotometric and multivariate spectrophotometric methods were described for the simultaneous determination of hydrochlorothiazide (HCTZ), hydralazine hydrochloride (H.HCl), and reserpine (RES) in combined pharmaceutical tablets. Methanol was used as a solvent for analysis and the whole UV region was scanned from 200-400 nm. The resolution was obtained by using multivariate methods such as the net analyte signal method (NAS), principal component analysis (PCA), and net analyte signal-principal component analysis (NAS-PCA) applied to the UV spectra of the mixture. The results obtained from all of the three methods were compared. NAS-PCA showed a lot of resolved data as compared to NAS and PCA. Thus, the NAS-PCA technique is a combination of NAS and PCA methods which is advantageous to obtain the information from overlapping results.
Keywords: Net Analyte Signal; Principal Component Analysis; Relative Standard Error of Prediction Net Analyte Signal; Principal Component Analysis; Relative Standard Error of Prediction

Share and Cite

MDPI and ACS Style

SHARMA, C.; BADYAL, P.N.; RAWAL, R.K. Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA. Sci. Pharm. 2015, 83, 599-610. https://doi.org/10.3797/scipharm.1505-03

AMA Style

SHARMA C, BADYAL PN, RAWAL RK. Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA. Scientia Pharmaceutica. 2015; 83(4):599-610. https://doi.org/10.3797/scipharm.1505-03

Chicago/Turabian Style

SHARMA, Chetan, Pragya Nand BADYAL, and Ravindra K. RAWAL. 2015. "Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA" Scientia Pharmaceutica 83, no. 4: 599-610. https://doi.org/10.3797/scipharm.1505-03

APA Style

SHARMA, C., BADYAL, P. N., & RAWAL, R. K. (2015). Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA. Scientia Pharmaceutica, 83(4), 599-610. https://doi.org/10.3797/scipharm.1505-03

Article Metrics

Back to TopTop