Next Article in Journal
Characterization of Oxidative Degradation Product of Darunavir by LC-MS/MS
Previous Article in Journal
Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Sci. Pharm.
Volume 83
Issue 4
10.3797/scipharm.1503-15
scipharm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2016). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Development of an Ion Chromatography Method for Analysis of Organic Anions (Fumarate, Oxalate, Succinate, and Tartrate) in Single Chromatographic Conditions

by
Yarbagi KAVIRAJ
1,2,*,
B. SRIKANTH
1,
J. MOSES BABU
1,
B. VENKATESWARA RAO
2 and
S. PAUL DOUGLAS
2
1
Analytical Research, Custom Pharmaceutical Services, Dr. Reddy’s Laboratories Ltd., Bollaram road, Miyapur, Hyderabad-500049 (AP), India
2
Department of Engineering Chemistry, Andhra University, Visakhapatnam-530003, India
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2015, 83(4), 611-622; https://doi.org/10.3797/scipharm.1503-15
Submission received: 24 March 2015 / Accepted: 29 May 2015 / Published: 29 May 2015
Versions Notes

Abstract

A single organic counterion analysis method was developed by using an ion chromatography separation technique and conductivity detector. This allows the rapid characterization of an API to support clinical studies and to fulfil the regulatory requirements for the quantitation of fumarate, oxalate, succinate, and tartrate counterions in active pharmaceutical ingredients (quetiapine fumarate, escitalopram oxalate, sumatriptan succinate, and tolterodine tartrate). The method was developed by using the Metrohm Metrosep A Supp 1 (250 × 4.0 mm, 5.0 µm particle size) column with a mobile phase containing an isocratic mixture of solution A: 7.5 mM sodium carbonate and 2.0 mM sodium bicarbonate in Milli-Q water and solution B: acetonitrile. The flow rate was set at 1.0 mL/min and the run time was 25 minutes. The developed method was validated as per ICH guidelines, and the method parameters were chosen to ensure the spontaneous quantitation of all four anions. The method was validated for all four anions to demonstrate the applicability of this method to common anions present in various APIs.
Keywords: Ion chromatography; Anions; Validation; Fumarate; Oxalate; Succinate; Tartrate Ion chromatography; Anions; Validation; Fumarate; Oxalate; Succinate; Tartrate

Share and Cite

MDPI and ACS Style

KAVIRAJ, Y.; SRIKANTH, B.; BABU, J.M.; RAO, B.V.; DOUGLAS, S.P. Development of an Ion Chromatography Method for Analysis of Organic Anions (Fumarate, Oxalate, Succinate, and Tartrate) in Single Chromatographic Conditions. Sci. Pharm. 2015, 83, 611-622. https://doi.org/10.3797/scipharm.1503-15

AMA Style

KAVIRAJ Y, SRIKANTH B, BABU JM, RAO BV, DOUGLAS SP. Development of an Ion Chromatography Method for Analysis of Organic Anions (Fumarate, Oxalate, Succinate, and Tartrate) in Single Chromatographic Conditions. Scientia Pharmaceutica. 2015; 83(4):611-622. https://doi.org/10.3797/scipharm.1503-15

Chicago/Turabian Style

KAVIRAJ, Yarbagi, B. SRIKANTH, J. MOSES BABU, B. VENKATESWARA RAO, and S. PAUL DOUGLAS. 2015. "Development of an Ion Chromatography Method for Analysis of Organic Anions (Fumarate, Oxalate, Succinate, and Tartrate) in Single Chromatographic Conditions" Scientia Pharmaceutica 83, no. 4: 611-622. https://doi.org/10.3797/scipharm.1503-15

APA Style

KAVIRAJ, Y., SRIKANTH, B., BABU, J. M., RAO, B. V., & DOUGLAS, S. P. (2015). Development of an Ion Chromatography Method for Analysis of Organic Anions (Fumarate, Oxalate, Succinate, and Tartrate) in Single Chromatographic Conditions. Scientia Pharmaceutica, 83(4), 611-622. https://doi.org/10.3797/scipharm.1503-15

Article Metrics

Back to TopTop