Next Article in Journal
Impact of Transgenic Arabidopsis thaliana Plants on Herbicide Isoproturon Phytoremediation through Expressing Human Cytochrome P450-1A2
Previous Article in Journal
Kin-Mediated Male Choice and Alternative Reproductive Tactics in Spider Mites
Article

A Four-Dimensional Organoid System to Visualize Cancer Cell Vascular Invasion

1
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan
2
Center of Molecular Innovation and Translational Research, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan
3
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
4
Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduated School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan
5
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 819-0395, Japan
*
Authors to whom correspondence should be addressed.
Biology 2020, 9(11), 361; https://doi.org/10.3390/biology9110361
Received: 5 September 2020 / Revised: 8 October 2020 / Accepted: 22 October 2020 / Published: 27 October 2020
(This article belongs to the Section Cancer Biology)
Using vascular organoid culture with collagen microfiber, we have established a method for culturing organoids that recapitulates the vascular invasion process of cancer cells. This culture model made it possible to four-dimensionally evaluate the dynamics of cancer cells infiltrating into blood vessels.
Vascular invasion of cancer is a critical step in cancer progression, but no drug has been developed to inhibit vascular invasion. To achieve the eradication of cancer metastasis, elucidation of the mechanism for vascular invasion and the development of innovative treatment methods are required. Here, a simple and reproducible vascular invasion model is established using a vascular organoid culture in a fibrin gel with collagen microfibers. Using this model, it was possible to observe and evaluate the cell dynamics and histological positional relationship of invasive cancer cells in four dimensions. Cancer-derived exosomes promoted the vascular invasion of cancer cells and loosened tight junctions in the vascular endothelium. As a new evaluation method, research using this vascular invasion mimic model will be advanced, and applications to the evaluation of the vascular invasion suppression effect of a drug are expected. View Full-Text
Keywords: vascular organoid; vascular invasion; mimic model; 4D culture; exosome; tight junction vascular organoid; vascular invasion; mimic model; 4D culture; exosome; tight junction
Show Figures

Figure 1

MDPI and ACS Style

Yanagisawa, K.; Konno, M.; Liu, H.; Irie, S.; Mizushima, T.; Mori, M.; Doki, Y.; Eguchi, H.; Matsusaki, M.; Ishii, H. A Four-Dimensional Organoid System to Visualize Cancer Cell Vascular Invasion. Biology 2020, 9, 361. https://doi.org/10.3390/biology9110361

AMA Style

Yanagisawa K, Konno M, Liu H, Irie S, Mizushima T, Mori M, Doki Y, Eguchi H, Matsusaki M, Ishii H. A Four-Dimensional Organoid System to Visualize Cancer Cell Vascular Invasion. Biology. 2020; 9(11):361. https://doi.org/10.3390/biology9110361

Chicago/Turabian Style

Yanagisawa, Kiminori, Masamitsu Konno, Hao Liu, Shinji Irie, Tsunekazu Mizushima, Masaki Mori, Yuichiro Doki, Hidetoshi Eguchi, Michiya Matsusaki, and Hideshi Ishii. 2020. "A Four-Dimensional Organoid System to Visualize Cancer Cell Vascular Invasion" Biology 9, no. 11: 361. https://doi.org/10.3390/biology9110361

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop