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Open AccessCommunication

Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected Bacillus Strains against Enterotoxigenic Escherichia coli

1
Department of Life Sciences, University of Roehampton, London SW154JD, UK
2
Animal Health Innovation, Chr. Hansen A/S, 2970 Hørsholm, Denmark
3
Departamento de Biología Molecular, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, Spain
*
Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Antibiotics 2020, 9(12), 849; https://doi.org/10.3390/antibiotics9120849
Received: 8 November 2020 / Revised: 24 November 2020 / Accepted: 25 November 2020 / Published: 27 November 2020
(This article belongs to the Section Novel Antimicrobial Agents)
Probiotics have been shown to bind to host receptors, which are important for pathogen adhesion and induce the host’s production of defence factors. They can activate the goblet-cell-derived production of mucins, a major component of the mucus layer and a physical barrier participating in limiting the proximity of microorganisms to the epithelial layer. In the last decade, Bacillus spp. strains have gained interest in human and animal health due to their tolerance and stability under gastrointestinal tract conditions. Moreover, Bacillus spp. strains can also produce various antimicrobial peptides that can support their use as commercial probiotic supplements and functional foods. The present study aimed to evaluate and determine the ability of selected Bacillus spp. strains to inhibit the growth of enterotoxigenic Escherichia coli (ETEC) F4 and to reduce binding of ETEC F4 to HT29-16E (mucus-secreting and goblet-like) human intestinal cells. Moreover, mucus production in the HT29 cells in the presence of the Bacillus spp. strains was quantified by ELISA. Bacillus spp. strains (CHCC 15076, CHCC 15516, CHCC 15541, and CHCC 16872) significantly inhibited the growth of ETEC F4. Moreover, the ability of the probiotic Bacillus spp. strains to stimulate mucin release was highly strain dependent. The treatment with Bacillus subtilis CHCC 15541 resulted in a significant increase of both MUC2 and MUC3 in HT29-16E cells. Therefore, this strain could be an up-and-coming candidate for developing commercial probiotic supplements to prevent infections caused by ETEC F4 and, potentially, other pathogens. View Full-Text
Keywords: Escherichia coli; adhesion; goblet-cell-derived mucins; probiotics; Bacillus Escherichia coli; adhesion; goblet-cell-derived mucins; probiotics; Bacillus
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MDPI and ACS Style

Bravo Santano, N.; Juncker Boll, E.; Catrine Capern, L.; Cieplak, T.M.; Keleszade, E.; Letek, M.; Costabile, A. Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected Bacillus Strains against Enterotoxigenic Escherichia coli. Antibiotics 2020, 9, 849. https://doi.org/10.3390/antibiotics9120849

AMA Style

Bravo Santano N, Juncker Boll E, Catrine Capern L, Cieplak TM, Keleszade E, Letek M, Costabile A. Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected Bacillus Strains against Enterotoxigenic Escherichia coli. Antibiotics. 2020; 9(12):849. https://doi.org/10.3390/antibiotics9120849

Chicago/Turabian Style

Bravo Santano, Natalia; Juncker Boll, Erik; Catrine Capern, Lena; Cieplak, Tomasz M.; Keleszade, Enver; Letek, Michal; Costabile, Adele. 2020. "Comparative Evaluation of the Antimicrobial and Mucus Induction Properties of Selected Bacillus Strains against Enterotoxigenic Escherichia coli" Antibiotics 9, no. 12: 849. https://doi.org/10.3390/antibiotics9120849

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