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Open AccessArticle

Comparative Transcriptome Analysis of Streptomyces Clavuligerus in Response to Favorable and Restrictive Nutritional Conditions

1
Grupo de Bioprocesos, Universidad de Antioquia, Calle, Medellín 70 No. 52–21, Colombia
2
Department of Microbial Sciences, School of Biosciences and Medicine, University of Surrey, Guildford GU2 7XH, UK
3
Group of Parasitology, School of Medicine, Universidad de Antioquia, Calle, Medellín 70 No. 52-21, Colombia
4
Grupo de Bioprocesos, Departamento de Ingeniería Química, Universidad de Antioquia, Calle, Medellín 70 No. 52-21, Colombia
*
Author to whom correspondence should be addressed.
Antibiotics 2019, 8(3), 96; https://doi.org/10.3390/antibiotics8030096
Received: 18 May 2019 / Revised: 5 July 2019 / Accepted: 9 July 2019 / Published: 19 July 2019
(This article belongs to the Special Issue Mechanism and Regulation of Antibiotic Synthesis in Streptomyces)
Background: Clavulanic acid (CA), a β-lactamase inhibitor, is industrially produced by the fermentation of Streptomyces clavuligerus. The efficiency of CA production is associated with media composition, culture conditions and physiological and genetic strain characteristics. However, the molecular pathways that govern CA regulation in S. clavuligerus remain unknown. Methods and Results: Here we used RNA-seq to perform a comparative transcriptome analysis of S. clavuligerus ATCC 27064 wild-type strain grown in both a favorable soybean-based medium and in limited media conditions to further contribute to the understanding of S. clavuligerus metabolism and its regulation. A total of 350 genes were found to be differentially expressed between conditions; 245 genes were up-regulated in favorable conditions compared to unfavorable. Conclusion: The up-regulated expression of many regulatory and biosynthetic CA genes was positively associated with the favorable complex media condition along with pleiotropic regulators, including proteases and some genes whose biological function have not been previously reported. Knowledge from differences between transcriptomes from complex/defined media represents an advance in the understanding of regulatory paths involved in S. clavuligerus’ metabolic response, enabling the rational design of future experiments. View Full-Text
Keywords: transcriptomic analysis; Streptomyces clavuligerus; gene expression; clavulanic acid; complex media; gene cluster transcriptomic analysis; Streptomyces clavuligerus; gene expression; clavulanic acid; complex media; gene cluster
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MDPI and ACS Style

Pinilla, L.; Toro, L.F.; Laing, E.; Alzate, J.F.; Ríos-Estepa, R. Comparative Transcriptome Analysis of Streptomyces Clavuligerus in Response to Favorable and Restrictive Nutritional Conditions. Antibiotics 2019, 8, 96.

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