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Antibiotics 2018, 7(4), 91; https://doi.org/10.3390/antibiotics7040091

Microbiome Analysis of Biofilms of Silver Nanoparticle-Dispersed Silane-Based Coated Carbon Steel Using a Next-Generation Sequencing Technique

1
Department of Chemistry and Biochemistry, National Institute of Technology, Suzuka College, Suzuka 510-0294, Japan
2
D&D Corporation, Yokkaichi 512-1211, Japan
3
Department of Material Science and Engineering, National Institute of Technology, Suzuka College, Suzuka 510-0294, Japan
4
Department of Marine Technology, National Institute of Technology, Toba College, Toba 517-8501, Japan
5
Department of Applied Chemistry and Biochemistry, University of Fukui, Fukui 910-8507, Japan
*
Author to whom correspondence should be addressed.
Received: 16 August 2018 / Revised: 25 September 2018 / Accepted: 16 October 2018 / Published: 22 October 2018
(This article belongs to the Special Issue Silver-Based Antimicrobials)
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Abstract

Previously, we demonstrated that silver nanoparticle-dispersed silane-based coating could inhibit biofilm formation in conditions where seawater was used as a bacterial source and circulated in a closed laboratory biofilm reactor. However, it is still unclear whether the microbiome of a biofilm of silver nanoparticle-dispersed silane-based coating samples (Ag) differs from that of a biofilm of non-dispersed silane-based coating samples (Non-Ag). This study aimed to perform a microbiome analysis of the biofilms grown on the aforementioned coatings using a next-generation sequencing (NGS) technique. For this, a biofilm formation test was conducted by allowing seawater to flow through a closed laboratory biofilm reactor; subsequently, DNAs extracted from the biofilms of Ag and Non-Ag were used to prepare 16S rRNA amplicon libraries to analyze the microbiomes by NGS. Results of the operational taxonomy unit indicated that the biofilms of Non-Ag and Ag comprised one and no phyla of archaea, respectively, whereas Proteobacteria was the dominant phylum for both biofilms. Additionally, in both biofilms, Non-Ag and Ag, Marinomonas was the primary bacterial group involved in early stage biofilm formation, whereas Anaerospora was primarily involved in late-stage biofilm formation. These results indicate that silver nanoparticles will be unrelated to the bacterial composition of biofilms on the surface of silane-based coatings, while they control biofilm formation there. View Full-Text
Keywords: biofilm; microbiomes; silver nanoparticles; silane-based coating; Marinomonas; Anaerospora biofilm; microbiomes; silver nanoparticles; silane-based coating; Marinomonas; Anaerospora
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Ogawa, A.; Takakura, K.; Sano, K.; Kanematsu, H.; Yamano, T.; Saishin, T.; Terada, S. Microbiome Analysis of Biofilms of Silver Nanoparticle-Dispersed Silane-Based Coated Carbon Steel Using a Next-Generation Sequencing Technique. Antibiotics 2018, 7, 91.

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