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Antibiotics 2018, 7(4), 92; https://doi.org/10.3390/antibiotics7040092

Explorative Study on Isolation and Characterization of a Microviridae G4 Bacteriophage, EMCL318, against Multi-Drug-resistant Escherichia coli 15-318

1
Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC V2C 0C8, Canada
2
Medical School, Karl Landsteiner Private University for Health Sciences, Dr. Karl Dorrek Strasse 30, A-3500 Krems an der Donau, Austria
3
Fort St. John Hospital Laboratory, Fort St. John, BC V1J 2A4, Canada
4
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Blvd, 5.104D Basic Science Building, Galveston, TX 77555, USA
*
Authors to whom correspondence should be addressed.
Received: 29 September 2018 / Revised: 21 October 2018 / Accepted: 24 October 2018 / Published: 26 October 2018
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Abstract

Bacteriophages screened and isolated from sewage water samples exhibited antibacterial activities against multi-drug-resistant Escherichia coli strains. Five different water samples from Canadian habitats such as Kamloops Wastewater Treatment Center, Domtar, the Pacific Ocean, Bisaro Anima Cave, and alkali ponds, were used in this study. Four Enterobacteriaceae strains including one non-resistant and three clinical multi-drug Escherichia coli strains (E. coli 15-102, E. coli 15-124, and E. coli 15-318) were selected as target bacteria to screen for the bacteriophages from these collected water samples. Seeded agar assay technique was implemented for the screening. It was found that only sewage water sample exhibited a significant number of plaques count with the E. coli 15-318 (1.82 × 102 plaques/plate) cells in comparison to E. coli non-resistant strain K12 (8 plaques/plate). The phage did not produce plaques in the E. coli 15-124 and E. coli 15-102 strains. The bacteriophage, designated EMCL318, was isolated, purified, characterized, and identified to belong to the G4 species of the Family Microviridae, GenBank accession number MG563770. This is an explorative study conducted in order to reveal the viruses as alternative potentials to fight against emerging and existing multi-drug-resistant infectious diseases. View Full-Text
Keywords: Multi-drug-resistant organisms; bacteriophages; phage therapy; alternative therapeutic measures Multi-drug-resistant organisms; bacteriophages; phage therapy; alternative therapeutic measures
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ghosh, S.; Persad, E.; Shiue, T.-Y.; Lam, C.; Islam, A.; Mascibroda, L.G.; Sherman, M.B.; Smith, T.; Cheeptham, N. Explorative Study on Isolation and Characterization of a Microviridae G4 Bacteriophage, EMCL318, against Multi-Drug-resistant Escherichia coli 15-318. Antibiotics 2018, 7, 92.

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