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Article

New Insight on Antibiotic Resistance and Virulence of Escherichia coli from Municipal and Animal Wastewater

1
The University of Veterinary Medicine and Pharmacy in Košice, Komenského 87, 040 01 Košice, Slovakia
2
Centre of Biosciences, Slovak Academy of Sciences, Institute of Animal Physiology, Šoltésovej 4, 040 01 Košice, Slovakia
*
Author to whom correspondence should be addressed.
Academic Editor: Lucia Bírošová
Antibiotics 2021, 10(9), 1111; https://doi.org/10.3390/antibiotics10091111
Received: 16 August 2021 / Revised: 10 September 2021 / Accepted: 13 September 2021 / Published: 14 September 2021
(This article belongs to the Special Issue Antibiotic Resistance in Wastewater and Its Treatment)
Antibiotic resistance of the indicator microorganism Escherichia coli was investigated in isolates from samples collected during the course of one year from two wastewater treatment plants treating municipal and animal wastes in Slovakia, respectively. The genes of antibiotic resistance and virulence factors in selected resistant E. coli isolates were described. A high percentage of the isolates from municipal and animal wastewater were resistant to ampicillin, streptomycin, tetracycline, ceftiofur, ceftriaxone, and enrofloxacin. In the selected E. coli isolates, we detected the following phenotypes: ESBL (20.4% in animal wastewater; 7.7% in municipal wastewater), multidrug-resistant (17% of animal and 32% of municipal isolates), high resistance to quinolones (25% of animal and 48% of municipal samples), and CTX-M (7.9% of animal and 17.3% of municipal isolates). We confirmed an integro-mediated antibiotic resistance in 13 E. coli strains from municipal and animal wastewater samples, of which the Tn3 gene and virulence genes cvaC, iutA, iss, ibeA, kps, and papC were detected in six isolates. One of the strains of pathogenic E. coli from the animal wastewater contained genes ibeA with papC, iss, kpsII, Int1, Tn3, and Cit. In addition, one blaIMP gene was found in the municipal wastewater sample. This emphasises the importance of using the appropriate treatment methods to reduce the counts of antibiotic-resistant microorganisms in wastewater effluent. View Full-Text
Keywords: E. coli; animal wastewater; municipal wastewater; resistance; ESBL E. coli; animal wastewater; municipal wastewater; resistance; ESBL
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MDPI and ACS Style

Gregova, G.; Kmet, V.; Szaboova, T. New Insight on Antibiotic Resistance and Virulence of Escherichia coli from Municipal and Animal Wastewater. Antibiotics 2021, 10, 1111. https://doi.org/10.3390/antibiotics10091111

AMA Style

Gregova G, Kmet V, Szaboova T. New Insight on Antibiotic Resistance and Virulence of Escherichia coli from Municipal and Animal Wastewater. Antibiotics. 2021; 10(9):1111. https://doi.org/10.3390/antibiotics10091111

Chicago/Turabian Style

Gregova, Gabriela, Vladimir Kmet, and Tatiana Szaboova. 2021. "New Insight on Antibiotic Resistance and Virulence of Escherichia coli from Municipal and Animal Wastewater" Antibiotics 10, no. 9: 1111. https://doi.org/10.3390/antibiotics10091111

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