Next Article in Journal
Optical Biosensors for Therapeutic Drug Monitoring
Previous Article in Journal
Review of Electroanalytical-Based Approaches for the Determination of Benzodiazepines
Open AccessArticle

Fully Automated Microsystem for Unmediated Electrochemical Characterization, Visualization and Monitoring of Bacteria on Solid Media; E. coli K-12: A Case Study

1
CMUA. Department of Electrical and Electronic Engineering, Universidad de los Andes, Carrera 1E # 19A-40, Bogota 111711, Colombia
2
Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, S-58183 Linköping, Sweden
3
Department of Printed Electronics, RISE Acreo, Research Institute of Sweden, 16440 Norrköping, Sweden
*
Author to whom correspondence should be addressed.
Biosensors 2019, 9(4), 131; https://doi.org/10.3390/bios9040131
Received: 13 August 2019 / Revised: 14 October 2019 / Accepted: 18 October 2019 / Published: 4 November 2019
In this paper, we present a non-fluidic microsystem for the simultaneous visualization and electrochemical evaluation of confined, growing bacteria on solid media. Using a completely automated platform, real-time monitoring of bacterial and image-based computer characterization of growth were performed. Electrochemical tests, using Escherichia coli K-12 as the model microorganism, revealed the development of a faradaic process at the bacteria–microelectrode interface inside the microsystem, as implied by cyclic voltammetry and electrochemical impedance spectrometry measurements. The electrochemical information was used to determine the moment in which bacteria colonized the electrode-enabled area of the microsystem. This microsystem shows potential advantages for long-term electrochemical monitoring of the extracellular environment of cell culture and has been designed using readily available technologies that can be easily integrated in routine protocols. Complementarily, these methods can help elucidate fundamental questions of the electron transfer of bacterial cultures and are potentially feasible to be integrated into current characterization techniques. View Full-Text
Keywords: bio-electrochemical systems; microsystem; bacteria; direct electron transfer; cyclic voltammetry; electrochemical impedance spectrometry; microscopy; automated system bio-electrochemical systems; microsystem; bacteria; direct electron transfer; cyclic voltammetry; electrochemical impedance spectrometry; microscopy; automated system
Show Figures

Graphical abstract

MDPI and ACS Style

Hernandez, C.A.; Beni, V.; Osma, J.F. Fully Automated Microsystem for Unmediated Electrochemical Characterization, Visualization and Monitoring of Bacteria on Solid Media; E. coli K-12: A Case Study. Biosensors 2019, 9, 131.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop