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Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor

1
Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Six Metrotech Center, Brooklyn, NY 11201, USA
2
State Key Laboratory of Precision Measuring Technology & Instruments, School of Precision Instruments and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China
*
Author to whom correspondence should be addressed.
Biosensors 2019, 9(2), 50; https://doi.org/10.3390/bios9020050
Received: 22 February 2019 / Revised: 26 March 2019 / Accepted: 29 March 2019 / Published: 31 March 2019
(This article belongs to the Special Issue Enzymatic Electrochemical Biosensors)
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Abstract

A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (>50 μM) and a wide linear range after optimization with AuNP electrodeposition. View Full-Text
Keywords: electrodeposition; potentiometry; gold nanoparticle; nanoparticle size; protein binding; enzyme activity; optimization electrodeposition; potentiometry; gold nanoparticle; nanoparticle size; protein binding; enzyme activity; optimization
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Chiang, H.-C.; Wang, Y.; Zhang, Q.; Levon, K. Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor. Biosensors 2019, 9, 50.

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