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Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules

1
School of Biomedical Engineering, University of Western Ontario, 1151 Richmond St., London, ON N6A 5B9, Canada
2
Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, ON N6A 5B9, Canada
*
Author to whom correspondence should be addressed.
Biosensors 2019, 9(1), 42; https://doi.org/10.3390/bios9010042
Received: 3 February 2019 / Revised: 9 March 2019 / Accepted: 12 March 2019 / Published: 16 March 2019
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Abstract

Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules. BRET is a distance dependent, non-radiative energy transfer, which uses a bioluminescent protein to excite an acceptor through the resonance energy transfer. A BRET sensor can quickly detect the change of a target biomolecule quantitatively without an external electromagnetic field, e.g., UV light, which normally can damage tissue. Having been developed quite recently, this technique has evolved rapidly. Here, different bioluminescent proteins have been reviewed. In addition to a multitude of bioluminescent proteins, this manuscript focuses on the recent development of BRET sensors by utilizing quantum dots. The special size-dependent properties of quantum dots have made the BRET sensing technique attractive for the real-time monitoring of the changes of target molecules and bioimaging in vivo. This review offers a look into the basis of the technique, donor/acceptor pairs, experimental applications and prospects. View Full-Text
Keywords: bioluminescence resonance energy transfer; fluorescent nanomaterials; fluorescent nanobiosensors; quantum dots bioluminescence resonance energy transfer; fluorescent nanomaterials; fluorescent nanobiosensors; quantum dots
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Hwang, E.; Song, J.; Zhang, J. Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules. Biosensors 2019, 9, 42.

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